By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same strand but 20 bases apart. Which of the following best describes how your sequence will look? A. The sequence will be readable for the first 20 bases only. B. The detector will detect two nucleotides at about 75% of the positions. C. The sequence will be readable for the last 20 bases only. D. The detector will detect one nucleotide at about 75% of the positions. E. The detector will detect no nucleotides at any of the positions.

Q: A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the…

A: if we cut the plasmid with XhoI and XbaI then we will get three fragments of following sizes  1000…

Q: A piece of linear DNA is 700000 base pairs in length and is digested with a restriction enzyme that…

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Q: A researcher is interested in using the in vitro technique of bisulfite conversion to confirm the…

A: DNA methylation is a chemical change that frequently takes place in the setting of CpG dinucleotides…

Q: Overall, a molecule of DNA does not have a positive or negtive charge since the bases are…

A: DNA stands for deoxyribonucleic acid. It is the genetic material of the cell. It is present in the…

Q: Why is it important that the alcohol used in the DNA extraction is kept cold? The solubility of any…

A: DNA can be extracted from any tissue or cell. There are four basic steps of DNA extraction. They…

Q: perform an experiment to determine the complete melting profile of a relatively long DNA duplex with…

A: Melting temperature is the temperature at which half of the double stranded DNA gets melted out…

Q: Read up on the latest developments in DNA sequencing, and present a summary of any one such…

A: DNA (deoxyribonucleic acid) sequencing is the technique of identifying or determining the order of…

Q: During a typical gel electrophoresis set up (negative pole at the top), which DNA fragment would be…

A: Gel electrophoresis is the technique for the separation of DNA fragments on the basis of size and…

Q: 2) A scientist wants to increase the amount of sample of a DNA sequence of 100 bps. Calculate the…

A: Given that molar mass of average DNA base pair is 600g/mol. That means 600g of base pairs contain…

Q: Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures?   a. Chain…

A: An oligonucleotide primer is annealed to a single-stranded DNA template and expanded by DNA…

Q: Gel electrophoresis can be used to separate DNA on the basis of:   A. the probe used.   B. size.…

A: Electrophoresis is a laboratory technique used to separate DNA, RNA or protein molecules. Agarose…

Q: In automated sequencing, you are given a printout of the sense strand of your DNA.  The printout is…

A: Introduction Nucleic Acids:- Nucleic acids are biopolymers, macromolecules, essential to all known…

Q: Which one of the following options would be a good way to identify the location of the poly A tail…

A: Pre mRNA undergoes several post-transcriptional modifications. One of these modifications is…

Q: if You have been transported to a DNA Lab by a mad scientist.    The scientist hands you a gel…

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Q: In order to determine the purity of a DNA sample, spectrophotometry can be carried our at Answer to…

A: Spectrophotometry is the quantitative analysis of spectra to compare the relative absorption or…

Q: What is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific…

A: Nucleic acids are large molecules that are made up of nucleotides that performs a various function…

Q: Write the nucleotide sequence, including the primer, in the 3'to 5' direction. 3'- -5'

A: The current iteration of the dideoxy chain termination sequencing technique is dye terminator…

Q: Place the steps of sanger sequencing in order. A.  A fluorescent laser excites the fragments and…

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Q: To be able to properly analyze a sample of DNA, you need to have at least 1 µg of the DNA of…

A: The polymerase chain reaction (PCR) is a molecular biology technique for amplifying particular…

Q: Shown below are several next-generation sequencing reads from a sample you have. Which of the…

A: Genomic research has been revolutionised by next-generation sequencing (NGS), commonly referred to…

Q: If you wanted to create recombinant DNA using this enzyme(3' T A 5'), would you have to cut both…

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Q: Certain restriction endonucleases produce cohesive (sticky) ends.  This means that they: a. stick…

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Q: How would you approach this problem? You plan to sequence the following DNA by Sanger sequencing.…

A: Sanger sequencing, developed by Frederick Sanger in 1977, is a widely used method for determining…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.