By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same strand but 20 bases apart. Which of the following best describes how your sequence will look? A. The sequence will be readable for the first 20 bases only. B. The detector will detect two nucleotides at about 75% of the positions. C. The sequence will be readable for the last 20 bases only. D. The detector will detect one nucleotide at about 75% of the positions. E. The detector will detect no nucleotides at any of the positions.
By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same strand but 20 bases apart. Which of the following best describes how your sequence will look? A. The sequence will be readable for the first 20 bases only. B. The detector will detect two nucleotides at about 75% of the positions. C. The sequence will be readable for the last 20 bases only. D. The detector will detect one nucleotide at about 75% of the positions. E. The detector will detect no nucleotides at any of the positions.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same strand but 20 bases apart. Which of the following best describes how your sequence will look?
A. The sequence will be readable for the first 20 bases only.
B. The detector will detect two nucleotides at about 75% of the positions.
C. The sequence will be readable for the last 20 bases only.
D. The detector will detect one
E. The detector will detect no nucleotides at any of the positions.
Explain why it is B.
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