Some restriction enzymes produce DNA fragments with overhanging stretches called sticky ends on each strand.  Sticky ends are useful in making recombinant DNA because Select one: a.  Sticky ends contain the exact same nucleotides that allows fragments to splice together. b.  Sticky ends contain nucleotides with complementary bases that allows fragments to splice together. c. Sticky ends contain the exact same nucleotides that can form hydrogen bonds. d. Sticky ends contain nucleotides with complementary bases that can form hydrogen bonds.

Q: 5) PCR primers have a melting temperature (Tm) at which they become dissociated from the template…

A: A primer is a short strand of nucleotide molecules that serves as an initial point for the synthesis…

Q: Match the terms associated with tne polymerase chain reaction with their correct descriptions.

A: PCR is the polymerase chain reaction in which Denaturation, Annealing and Extension process takes…

Q: For the statements below, indicate whether the statement applies to the leading strand, or to the…

A: 1. Deoxyribonucleic acid (DNA) replication is the process in which new complementary DNA strands are…

Q: A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the…

A: if we cut the plasmid with XhoI and XbaI then we will get three fragments of following sizes  1000…

Q: Overall, a molecule of DNA does not have a positive or negtive charge since the bases are…

A: DNA stands for deoxyribonucleic acid. It is the genetic material of the cell. It is present in the…

Q: How many subunits does the E. coli DNA polymerase I have? Select one: O a. 5 Оb. 10 О с. 1 O d. 3

A: RNA polymerase is required for the transcription of DNA into RNA. It is also important in the study…

Q: DNA synthesis has a very low error rate.  One reason for this is that the DNA polymerase enzyme can…

A: DNA polymerase is the enzyme that does maximum of the work throughout DNA replication. It builds a…

Q: How many fragments would be produced if the DNA is cut by that enzyme?    Number each fragment…

A: * Restriction enzymes recognizes specific palindrome sequence and cut DNA only at that specific…

Q: During DNA replication, the Okazaki fragments are Select one: O a. single-strand RNA O b. RNA-DNA…

A: Option c

Q: You have a DNA sequence of 1800 bp. To characterize it, you decide to make a restriction map.…

A: Restriction mapping is a process that helps to determine the location of the restriction site. The…

Q: DNA polymerases make errors in matching as DNA is synthesized. These errors can be detected and…

A: DNA replication is the process by which two exact copies of DNA are made from an original DNA…

Q: Name three intermolecular forces that stabilize the structure of DNA, and explain how they act. A.…

A: DNA, or deoxyribonucleic acid, is a molecule that carries the genetic instructions used in the…

Q: If DNA had 2 different bases, a restriction enzyme with a 4-base recognition sequence would cut DNA…

A: The frequency of cutting in a random DNA sequence for a given restriction enzyme is once per every…

Q: What can cause mutations in the DNA sequence? Select all that apply.   A. Failure of repair enzymes…

A: Answer: Introduction: Mutation- These are the random heritable changes that occurs in the DNA…

Q: Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures?   a. Chain…

A: An oligonucleotide primer is annealed to a single-stranded DNA template and expanded by DNA…

Q: A researcher sequences the genome of a variety of bacterial and eukaryotic cells. She finds that the…

A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…

Q: One way scientists determine the abundance of different DNA molecules in a sample is by measuring…

A: Polymerase chain reactionPCR is a rapid and versatile in vitro method for amplifying defined target…

Q: The DNA polymerase cannot start making a new DNA polymer out of nothing, it needs preexisting…

A: DNA molecules makes their own identical copies by a process known as DNA replication. It takes…

Q: By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same…

A: The correct answer is:B. The detector will detect two nucleotides at about 75% of the positions.

Q: Which of the following is NOT true of DNA polymerase?   a.  DNA polymerase builds the new strand…

A: DNA polymerase is a type of enzyme found in all living things. Its primary function is to duplicate…

Q: Which of the following molecules helps relieve the tension of unwinding parental DNA strands, by…

A: DNA replication is defined as a process by which a single DNA molecule will produce two identical…

Q: The RNA primer at the beginning of each Okazaki fragment is removed by

A: Introduction: The small pieces of the discontinuously synthesized DNA are basically known as Okazaki…

Q: DNA damage by uv light creates pyrimidine dimers between adjacent pyrimidines on the same strand.…

A: DNA damage is defined as a change in the basic structure of DNA that is not itself replicated when…

Q: DNA Polymerase holoenzymes used for DNA replication recognizes   A. double-stranded sequences as…

A: DNA polymerase III holoenzyme (Pol III HE) is an enzyme that catalyzes elongation of DNA chains…

Q: What is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific…

A: Nucleic acids are large molecules that are made up of nucleotides that performs a various function…

Q: listed below, match each to the correct definition. Definition a. Unwinds DNA b. Relieves tension in…

A: All these enzymes help and DNA replication. DNA replication process in which exact similar…

Q: Write the nucleotide sequence, including the primer, in the 3'to 5' direction. 3'- -5'

A: The current iteration of the dideoxy chain termination sequencing technique is dye terminator…

DNA and RNA

Deoxyribonucleic acid (DNA) is usually called the blueprint of life. Deoxyribose is a monosaccharide that has a key function in the synthesis of deoxyribonucleic acid. One less oxygen-containing hydroxyl group occurs in deoxyribose sugar. Nucleic acid, deoxyribonucleic acid, is one of the natural components. Deoxyribonucleic acid is a double-stranded molecule. Watson and Crick postulated the double-stranded model of the helix. A deoxyribonucleic acid is a molecular group that carries and transmits genetic information from parents to offspring. All eukaryotic and prokaryotic cells are involved.

DNA as the Genetic Material

DNA, or deoxyribonucleic acid, is a long polymeric nucleic acid molecule discovered in the late 1930s. It is a polymer; a long chain-like molecule made up of several monomers connected in a sequence. It possesses certain characteristics that qualify it as a genetic component. Certain organisms have different types of nucleic acids as their genetic material - DNA or RNA.

Genetics

The significant branch in science which involves the study of genes, gene variations, and the organism's heredity is known as genetics. It is also used to study the involvement of a gene or set of genes in the health of an individual and how it prevents several diseases in a human being. Thus, genetics also creates an understanding of various medical conditions.      

DNA Replication

The mechanism by which deoxyribonucleic acid (DNA) is capable of producing an exact copy of its own is defined as DNA replication. The DNA molecules utilize a semiconservative method for replication.