You are set to run your first SDS polyacrylamide-gel electrophoresis. You have boiled yoursamples of protein in SDS in the presence of mercaptoethanol and loaded them into the wellsof a polyacrylamide gel. You are now ready to attach the electrodes. Uh oh, does the positiveelectrode (the anode) go at the top of the gel, where you loaded your proteins, or at thebottom of the gel?

SDS-PAGE, which is an abbreviation for sodium dodecyl sulfate-polyacrylamide gel electrophoresis, is…

Answered: You are set to run your first SDS…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Determine the concentration of monocytogenes enumerated from the apple slice based on the information below. You must draw your serial dilution and show your mathematical calculation to receive full credit. The apple slice (12 g) was first mixed with 108 ml of sterile 0.1% peptone water. After, 1 ml was added to tube containing 9 ml of sterile 0.1% peptone water. Process was repeated with two additional tubes. 1 ml was pipetted from tubes 2 and 3 onto MOX media. Plates were incubated at 37 for 48 hours. The pictures below show the plates from tube 2 (left) and tube 3 (right).
You have begun your career in medicinal biochemistry and have just discovered a bacterial DNA plasmld (transferabl ring of DNA) that appears to destroy the Ebola virus. In order to characterize your new plasmid, the molar mass of the plasmid must be determined. You dissolve 25.00 mg of the purified plasmid in 0.200 mL of water at 2 °C and find the osmotlc pressure of this solution is 1.20 Torr at 20 °C and 1 atm pressure. Answer the following about the Ebola-killing plasmid. 33.) The osmotlc pressure of the system is: (a) 1 atm (b) 0.016 atm (c) 6.5 X 10-5 atm (d) 22.59 atm (e) 0.0016 atm
Nanoparticles were added in a liquid to prompt the medication release, and it was observed that 5 mg of the medication was released in 1 liter of PBS, which accounted for 60% of the total amount of the drug. While producing an equivalent number of nanoparticles, 5 ml of 10 mg ml−1 of the drug was added. What is the efficiency of encapsulation?
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Consider the following properties of the protein components of a sample mixture as provided in the table below. Protein Molecular IpH Percentage of polar amino acid residues Weight (kDa) (%) АСЕ 200 7 20 CLU 25 65 DIA 100 10 40 НЕА 50 80
After running our protein samples on an SDS-PAGE gel, we will use a Coomassie-based dye (Gel Code Blue) to stain the gel. Based on your prior experience with Coomassie dye and understanding of its function, what do you predict this dye to be able to stain? A) Only protein tags B) Only DHFR protein C) Only recombinant proteins D) All proteins
You're purifying some plasmid DNA from a culture of bacteria and you want to know how pure it is. You measure the optical density at 260 m and 280 m and find the ratio is 2.0. You suspect there is RNA contamination in your preparation, so you treat your preparation with RNase. But the ratio is still 2.0. Protein assays tell you there is no protein in your solution, and no other biological molecules absorb light very efficiently at those wavelengths. What's the explanation?
What do you mean by amphoteric substance? Give examples of substance that is/are amphoteric. What is the purpose of the power supply in gel electrophoresis? Among the short and long strand of DNA, which moves the farthest in the gel and why? What is the purpose of buffer in agarose gel electrophoresis?
The following gel was obtained using the Sanger method. What is the DNA sequence and what does the arrow represent?
Give Detailed Solution with explanation (no need Handwritten)
While working with eukaryotic cell cultures, certain requirements such as apparatuses, reagents, ethical approvals etc. are necessary. What are the requirements of a laboratory dedicated to animal cell culture experiments? As a cost effective replacement, what other types of organisms are being experimented upon for preparation of complex proteins?
Match the types of data to the example. Precise measurements Assay standard Positive control Quantitative results Negative control Qualitative results Standard curve…

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