Why shouldn’t a buffer with a pH of 10.0 be used as a mobile phase in an HPLC separation? Group of answer choices Basic pH’s never improve the separation of compounds. At pH’s above 8, the linkage holding the stationary phase on the silica particle degrades. Basic solutions will deprotonate a molecule which makes them impossible to elute from a column. Basic pH’s will cause peak tailing.

Why shouldn’t a buffer with a pH of 10.0 be used as a mobile phase in an HPLC separation? Group of answer choices Basic pH’s never improve the separation of compounds. At pH’s above 8, the linkage holding the stationary phase on the silica particle degrades. Basic solutions will deprotonate a molecule which makes them impossible to elute from a column. Basic pH’s will cause peak tailing.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.12QAP

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