Why do the results for the confirmed test represent faecal coliform in the analysis of water using the MPN procedure
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- Why do the results for the confirmed test represent faecal coliform in the analysis of water using the MPN procedure?
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- What is the purpose of the confirmed test in an experiment designed to test for coliform bacteria?If no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?Why does the oxidase test must be performed from blood agar or another medium without a fermentable sugar as MacConkey agar?
- When conducting the Triple Sugar Iron Test, what is the result of the bacteria Staphylococcus epidermidis?If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what is the approximate amount of ampicillin (grams) in each plate?If the five-digit code that is tabulated cannot be found in the EnteroPluri-Test Codebook, what that indicate about the bacterial culture?
- What is the dilution factor used for the glycogen isolate? Show complete solution.How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.You were instructed to add 1.0 mL out of 4.0 mL of an undiluted sample to 99 mL of sterile diluent. Instead, you add the entire 4.0 mL to the 99 mL. A.) What was your intended dilution factor? B.) What was your actual dilution factor?