Glycogen was isolated from a liver sample. Sixty milligrams of the crude glycogen were then dissolved in 10.0 mL distilled water. From this stock solution, 0.50 mL aliquot was taken and transferred onto a new tube. To this sample, all the reagents for acid hydrolysis. were added giving a total volume of 10.00 mL. A 0.50 mL was then taken and diluted with 0.50 mL of distilled water. The glucose content from the completely hydrolyzed glycogen was measured using Nelson's assay giving an absorbance of 0.390. Standard solutions were prepared by diluting each aliquot of glucose solution to 1.00 mL and then subjecting each to Nelson's method. The following data were obtained from this experiment.

Glycogen was isolated from a liver sample. Sixty milligrams of the crude glycogen were then dissolved in 10.0 mL distilled water. From this stock solution, 0.50 mL aliquot was taken and transferred onto a new tube. To this sample, all the reagents for acid hydrolysis. were added giving a total volume of 10.00 mL. A 0.50 mL was then taken and diluted with 0.50 mL of distilled water. The glucose content from the completely hydrolyzed glycogen was measured using Nelson's assay giving an absorbance of 0.390. Standard solutions were prepared by diluting each aliquot of glucose solution to 1.00 mL and then subjecting each to Nelson's method. The following data were obtained from this experiment.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

With the aid of a diagram, explain the principles of gel filtration chromatography.
In the Mannitol Salt Agar, why is there no observed growth of Escherichia coli?
Do the results from a confirmed coliform test represent total or faecal coliform and why?
Aside from Paraffin, what is your recommended infiltrating medium for routine purposes? why?
What is bactericide exposure?
Explain why it is important to wash the wells with PBS between the additions of each reagent?
Why is penicillin toxic to bacteria but not to higher organism? Explain briefly.
What kinds of organisms may be plentiful in a milk sample, yet give a negative reductase test?
how is the 0.5 McFarland standard prepared? What is the role of this standard in performing antimicrobial susceptibility test?
If testing had come back as oxidase negative, urease positive and growth on MacConkey agar, what closely related organism could be growing?
Why is cobalt chloride paper used in the experiments?
Why do we grow the bacteria in minimal media when testing for citrate utilization?