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Write the materials, reagents, Principle and procedure for the Periodic acid Schiff technique
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- Show workflow process of producing a Liquid Dosage Form through Extemporaneous CompoundingUse diagrams (boxes and arrows) to signify stepwise and significant procedures.Demonstrate the procedure for arriving at the answer. A. It is necessary to carry out baths with Amitraz 12.5% in a batch of 25 patients. The product label says to dilute 1.5 ml of the product in 1 liter of water, for this there is a 50-liter water tank to prepare the product. Each patient corresponds to 1.2 L of the product diluted in water for an effective treatment. Calculate: a) total ml of amitraz needed to make the dilution in the water tank. b) ml of the product for each patient. c) Total ml of diluted Amitraz for the batch of patients. Answer: R: a) 75 ml. b) 1200 ml. c) 30.000 ml (Ctrl)-Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.
- Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid Schiff technique 2. Grocott's hexamine silver technique 3. Ziehl-Neelsen stain 4. Lactophenol blue stain 5. Mehtamine silver technique 6. Shikata's orcein method 7. Mann's methyl blue-eosin stainIf you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.write the protocol for Hexamine silver staining, including their reagents, materials, prinicple, procedures results, and cautions
- Which is the better reagent for the clinical test between Fehling's solution and Benedict's solution? Explain briefly the answerWith the aid of diagrams, explain the processes and principles of each of the following methods: (a) Gel filtration chromatography on Sephadex G50 b.SDS-PAGEBradford method Reagents Stock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue G250 in a mixture consisting of 100ml of 85% phosphoric acid, 50ml of 95% ethanol and 50ml 1M NAOH. Store at 4°C until precipitation occurs, at which point it is discarded. Working Bradford reagent: Prepare fresh by diluting 10ml of stock Bradford reagent to 250ml with distilled water. Stock bovine serum albumin (BSA) solution (10mg/ml): Dissolve 0.2g BSA in 20ml distilled water. Working BSA concentration range: 0.5 – 1.25 mg/ml Method 1. Prepare the folloving test tubes: Table 1: Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Unknown Distilled H20 1.0 ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.25 mg/ml BSA 0.1 ml 0.5 mg/ml BSA 0.1 ml 0.75 mg/ml BSA 0.1 ml 1.0 mg/ml BSA 0.1 ml 1.25 mg/ml BSA 0. 1ml Unknown 0.1ml protein Working 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml Bradford 2. After each addition of the working Bradford reagent, thoroughly mix the…