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Illustrate the dilution series used and label the final dilution of each dilution.

Illustrate the dilution series used and label the final dilution of each dilution.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Illustrate the dilution series used and label the final dilution of each dilution.
1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225ml 0.90% NaCl. Three consectutive dilutions were further made by using 1ml aliquot in 9ml of 0.90% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating methods. Sampling COUNTS 1st dilution 2nd dilution 3rddilution 4th dilution 1st 54; 61 3; 7 0; 0 0,0 2nd TNTC TNTC 242; 233 28: 37 3rd TNTC TNTC TNTC 249;246 *TNTC = Too numerous to count
Transcribed Image Text:1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225ml 0.90% NaCl. Three consectutive dilutions were further made by using 1ml aliquot in 9ml of 0.90% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating methods. Sampling COUNTS 1st dilution 2nd dilution 3rddilution 4th dilution 1st 54; 61 3; 7 0; 0 0,0 2nd TNTC TNTC 242; 233 28: 37 3rd TNTC TNTC TNTC 249;246 *TNTC = Too numerous to count
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