Explain the chemical basis (reaction) of Millon’s test. (Generic reaction and explanation) What is the difference between Millon's test and Biuret test?(minimum 5)
Q: Compare and contrast Benedict’s Test, Reagent Test, and Iodine Test in terms of materials used
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Q: Polysaccharides generally do not give a positive test with Fehling’s solution. Why? *
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- Provide the Principles and detailed procedures of the following tests for the "Color Reaction of Proteins". 1. Hopkins-Cole Test 2. Sulfur Test 3. Heller's TestDraw/illustrate the chemical reaction involved in Lugol’s test and explain the purpose of this qualitative test?Results and Discussion: Folin's McCarthy Sullivan Test: Samples: proline, methionine, alanine Reagents: sodium hydroxide (5N), Glycine (1%) and 10% sodium nitroprusside solution, 6N HCl -To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (5N), followed by addition of few drops of glycine (1%) and 10% sodium nitroprusside solution (HANDLE WITH CARE)and vortex. Place the test tube in a hot water bath, maintained at 40 C, for 15 minutes. Cool the test tube in ice cold water for 5 minutes and add 0.5 ml of 6N HCl. Vortex the contents and allow to stant fpr 15 minutes at room temperature.
- The table below summarizes the results for Hopkins-Cole test. Provide the correct remarks from the results if Negative for Hopkin's Cole test or Positive for Hopkin's Cole test and if Negative for indole group or Has the presence of indole group.Qualitative and Quantitative Tests for Amino Acids and ProteinsComplete the table: Test Reagents Positive Result Positive Indication Ninhydrin test Xanthoproteic acid test Millon's test Hopkins Cole test Pauly's diazo Test Lead sulphide test Histidine test Isatin test Folin’s McCarthy Sullivan Test Nitroprusside TestParaphrase the text below: Series of test tubes were filled with the desired volume of the BSA (0.1, 0.2, 0.3… 1 ml) .PBS was added to this to make the volume of 1 mL. 5 mL of copper reagent was added to all the tubes. After proper mixing, all the tubes were incubated at room temperature for 15 minutes. 1 mL Folin reagent was added to each and mixed properly with the help of vortex mixer and incubated for 20 minutes. The intensity of the colour was then determined spectrophotometrically at 680 nm. The graph was than plotted between optical density and the amount of BSA. Proteins estimation was done for the detection for the amount of proteins present in the sample solutions by the Lowry method
- Provide a chemical test/reagent that would differentiate each of the following pairs of compounds. Also, for each test, indicate the compound (write only the corresponding CAPITAL LETTER) that would give the positive result, and the indication(s) for a positive result. ОН ОН 1. and A -CEC-H and 2. B HO CH3 OH 3. and B AThe reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…General Concept: Match each test with the substance detected Match each test with the substance it is intended to detect. 1 3 brown Paper test Benedict's lipids protein simple sugars 2 Match each of the options above to the items below. starch 4 iodine biuret test
- Demonstrate the procedure for arriving at the answer. A. It is necessary to carry out baths with Amitraz 12.5% in a batch of 25 patients. The product label says to dilute 1.5 ml of the product in 1 liter of water, for this there is a 50-liter water tank to prepare the product. Each patient corresponds to 1.2 L of the product diluted in water for an effective treatment. Calculate: a) total ml of amitraz needed to make the dilution in the water tank. b) ml of the product for each patient. c) Total ml of diluted Amitraz for the batch of patients. Answer: R: a) 75 ml. b) 1200 ml. c) 30.000 ml (Ctrl)-Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to Benedict’s test?What is the minimum volume of dissolution media required to provide sink condition in dissolution test of a tablet dosage form (its weight: 1350 mg; dose strength: 1000 mg) regarding the following conditions? The molecular weight of an active drug substance is 500 Da; the dissolution media is water and the solubility of active drug substance in water is 10 mg/Ml (4. SLAYT) 500 ml 1000 ml 1350 ml 100 ml 135 ml