Periodic acid Schiff technique detail  explanation

Q: Please provide in detail.

A: In liquid chromatography Elution is the process of separating or extracting material which get…

Q: Review method used to increase the solubility of a drug under the following headings co solvents PH…

A: Bioavailability is a powerful determinant of drug absorption. It represents the administered dose…

Q: Why are biochemical tests used to identify microbes?

A: Microbes are living organisms that are very small and can be seen only with the help of a…

Q: The Cavity Ring-Down Spectroscopy (CRDS) technique offers greater  sensitivity compared to other…

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Q: Give the principle involved in the ninhydrin test. What is its purpose?

A: The principle involved in the ninhydrin test is that the two molecules of ninhydrin react with the…

Q: Write the protocol for the periodic acid schiff technique

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Q: How to do cellulolytic characterization using a 96-well plate? What is the protocol or procedure to…

A: Cellulolytic action can be measured by an assortment of techniques that have been summed up in…

Q: Repeated freezing and thawing, sonication, French press, etc. are some of the methods for enzyme…

A: These steps are performed for cell disruption to release the desired biomolecules from cells. This…

Q: phenazopyridine

A: Phenazopyridine could be a pain reliever that affects the lower a part of your tract (bladder and…

Q: What does an area of clearing indicate in biochemical test?

A: Measuring a nutrient or its metabolite in the blood, faeces, urine, or other tissues that have a…

Q: List the methods/techniques used to enhance drug solubility and briefly describe each.

A: Introduction Solubility, the phenomenon of dissolving a solute in a solvent to produce a…

Q: Why did the blank used in experimental procedures contain substrate, buffer and water?

A: A blank or blank determination is an analysis without a sample (Enzyme). Any enzyme assay requires a…

Q: What are the disadvantages of biochemical test?

A: Biochemical tests refer to the test done to identify microorganisms. These tests are widely done in…

Q: Describe in detail the process of electrospray ionization ?

A: Ionisation is done by high voltage. Ions spread evenly on a surface. Ionised liquid gives rise to…

Q: Describe the dialysate solutions used in both procedures.

A: Dialysis is a treatment performed when the kidneys of a person are not able to remove excess fluid…

Q: Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors…

A: A bioreactor is a man-made device specially designed for supporting biologically active environment.…

Q: Please answer fast AFFINITY CHROMATOGRAPHY a) Explain the mechanism by which the CB column binds…

A: A) Affinity chromatography based upon - molecular interactions.  Affinity is indirectly proportional…

Q: 4) How would you perform a 10-fold dilution, if you want the final volume to be 9mL? What volume of…

A: Dilution is the process of decreasing the concentration of a solute in a solution. To dilute a…

Q: Table I. Sakaguchi Reaction Samples 1. Arginine 2. Casein Observation 3. Albumin 4. Gelatin 5.…

A: Sakaguchi test was a specific test for the presence of arginine amino acid. The main principle…

Q: Why is reproducibility of pipetting critical in performing serial dilutions?

A: Introduction :- The movement of liquids from one place to another would be risky and messy without a…

Q: Based on the experimental data provided, estimate the amount of amino acid in the given unknown…

A: Background information Ninhydrin is a strong oxidizing agent. It causes oxidative decarboxylation…

Q: Discuss the principles of Thiobarbituric reactive species (BARS) assay

A: Within a cell, reactive oxygen species (ROS) such as singlet oxygen, peroxides etc are produced as a…

Q: In the traditional alkaline lysis method of Solution 1, what is the purpose of Tris pH 8 with HCl?

A: The traditional alkaline lysis method is a widely used and classic technique for isolating plasmid…

Q: Streptavidin AP washing buffers purpose?

A: Streptavidin Alkaline Phosphatase (AP) washing buffers are essential for molecular biology and…

Q: Bio If you plated 100 uL of bacteria from a 10-5 dilution and count 73 colonies after incubating…

A: To calculate the CFU/mL (Colony-Forming Units per milliliter) of the original (undiluted) culture,…

Q: Aside from ovalbumin, what are other possible proteins which can be isolated from egg white? Give…

A: Proteins are the polymers of amino acids, white egg is the liquid which is present within an egg. It…

Q: Protocol for Methamine silver technique

A: Methamine Silver Staining is a staining method that is used in histology to check the presence of…

Q: What advantage(s) does nucleic acid amplification have overstandard culture methods for…

A: The identification of microbes in the patient sample can be done through culture based or…

Q: Explain this State Downstream processing will primarily be concerned with initial separation of the…

A: When under highly specified conditions, mass culturing of microorganisms is done to obtain a…

Q: Biochemical tests such as the fermentation test can be used when unknown bacterial cells are…

A: Fermentation Test: Fermentation tests are a type of biochemical test used to identify bacterial…

Q: Explain the extraction method and the procedure for extraction of Echinolone by using…

A: Chromatography works on the principle of molecules in a mixture being applied to a surface or a…

Q: Why

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Q: When preparing samples for SDS-PAGE, what is the purpose of boiling the samples at 95°C?

A: Electrophoresis is a laboratory technique used to separate and isolate desired nucleic acid…

Q: how is it possible to isolate both aerobes and anaerobes using streak plates?

A: The streak plate method is implemented for the isolation of anaerobic or aerobic bacteria from a…

Q: Explain why protein purification procedures: normally carried out 4-5°C. are

A: Crude extracts  that contain the protein of interest should be refrigerated, and the recombinant…

Q: mportance of pH adjustment in culture media?

A: Culture media is also known as growth media. It is a mixture of nutrients that helps for the growth…

Q: Step-by-step smear preparation of Kinyoun Method

A: Kinyoun, is a process used to stain acid-fast species of the bacterial genus Mycobacterium. it is a…

Q: Why do we add powdered zinc to the nitrate reduction test?

A: Bacteria are prokaryotic simple unicellular organisms. Their mode of respiration may be aerobic or…