Answered: Explain the mechanism leading to the…

Explain the mechanism leading to the given reactions in the inoculated biochemical media. 1. Blackening of the medium in Triple Sugar Iron Test slant

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter42: Basic Microbiology

Section: Chapter Questions

Problem 4CR

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Question

Explain the mechanism leading to the given reactions in the inoculated biochemical media.

1. Blackening of the medium in Triple Sugar Iron Test slant
2. Development of pink to red color in nitrate broth in Nitrate Reduction Test

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CITRATE TESTThis test determines the ability of bacteria to use citrate as source of carbon andinorganic ammonium salt as a source of nitrogen. The following are the stepwise procedurein conducting citrate test:1. Infuse the Simmons citrate agar on the slant by touching the tip of sterilized needleto a colony.2. Incubate at 37 degree Celsius for 24 to 48 hours.A positive result is indicated by the pH indicator, bromothymol blue which turns the growthgreen to blue. Task: In a tabulated form, determine if Salmonella typhi the are positive or negative withcitrate test. Interpret your answer and include the reaction of organism on this test.
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