Which of the following statements in incorrect? O in affinity chromatography a ligand is secured to the column matrix that will associate with a protein of interest O separation of proteins by non-denaturing PAGE will not accurately reflect the size of proteins O lon exchange chromatography only works to separate proteins when the pH of the elution buffer is equal to the pl of the proteins O In ion exchange chromatography, the bound proteins are eluted from the column using a high salt concentration solution

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Which of the following statements in incorrect?
O in affinity chromatography a ligand is secured to the column matrix that will associate with a protein of interest
O separation of proteins by non-denaturing PAGE will not accurately reflect the size of proteins
O lon exchange chromatography only works to separate proteins when the pH of the elution buffer Iis equal to the
pl of the proteins
O In ion exchange chromatography, the bound proteins are eluted from the column using a high salt concentration
solution
Transcribed Image Text:Which of the following statements in incorrect? O in affinity chromatography a ligand is secured to the column matrix that will associate with a protein of interest O separation of proteins by non-denaturing PAGE will not accurately reflect the size of proteins O lon exchange chromatography only works to separate proteins when the pH of the elution buffer Iis equal to the pl of the proteins O In ion exchange chromatography, the bound proteins are eluted from the column using a high salt concentration solution
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