Lysozyme purified by ion-exchange chromatography was collected from 380 mL egg white. i) The initial rate of the reaction catalyzed by 0.15 mL of 50-fold diluted egg white was 0.320 OD unit min-¹. ii) The initial rate of the reaction catalyzed by 0.15 mL of 20-fold diluted pH 10.5 filtrate was 0.900 OD unit min-¹. If there were 60 mLs of pH 10.5 filtrate collected, what percentage of original activity for egg white was recovered? Show all calculations.

Lysozyme purified by ion-exchange chromatography was collected from 380 mL egg white. i) The initial rate of the reaction catalyzed by 0.15 mL of 50-fold diluted egg white was 0.320 OD unit min-¹. ii) The initial rate of the reaction catalyzed by 0.15 mL of 20-fold diluted pH 10.5 filtrate was 0.900 OD unit min-¹. If there were 60 mLs of pH 10.5 filtrate collected, what percentage of original activity for egg white was recovered? Show all calculations.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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