Lysozyme purified by ion-exchange chromatography was collected from 380 mL egg white. i) The initial rate of the reaction catalyzed by 0.15 mL of 50-fold diluted egg white was 0.320 OD unit min-¹. ii) The initial rate of the reaction catalyzed by 0.15 mL of 20-fold diluted pH 10.5 filtrate was 0.900 OD unit min-¹. If there were 60 mLs of pH 10.5 filtrate collected, what percentage of original activity for egg white was recovered? Show all calculations.

Lysozyme purified by ion-exchange chromatography was collected from 380 mL egg white. i) The initial rate of the reaction catalyzed by 0.15 mL of 50-fold diluted egg white was 0.320 OD unit min-¹. ii) The initial rate of the reaction catalyzed by 0.15 mL of 20-fold diluted pH 10.5 filtrate was 0.900 OD unit min-¹. If there were 60 mLs of pH 10.5 filtrate collected, what percentage of original activity for egg white was recovered? Show all calculations.

Chemistry

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ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

Lysozyme purified by ion-exchange
chromatography was collected from 380
mL egg white.
i) The initial rate of the reaction catalyzed
by 0.15 mL of 50-fold diluted egg white was
0.320 OD unit min-1.
ii) The initial rate of the reaction catalyzed
by 0.15 mL of 20-fold diluted pH 10.5 filtrate
was 0.900 OD unit min-.
If there were 60 mLs of pH 10.5 filtrate
collected, what percentage of original
activity for egg white was recovered? Show
all calculations.

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