Q: name the six different types of vectors?
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Q: We have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed.…
A: Introduction The non-sexual flow of genetic information between genomes is known as horizontal gene…
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Q: The mechanism of specialized transduction relies on the improper excision of a bacteriophage genome…
A: Gene transfer is the method of inserting genetic material (DNA or a gene of interest) into cells…
Q: Generally a plasmid vector contains how many elements?a) 1b) 2c) 3d) 4
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Q: protien gradient formed
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Q: Using the information provided below, which of the individuals A and B have: i) Genotypic change i)…
A: DNA is made up of four nucleotides A, T, C and G. Mutation is the change in the genetic sequence or…
Q: Which of the following contain only the sequences required for transposition and the genes for…
A: Transposons are the DNA sequence which can change their position within the genome. It may be simple…
Q: Which of the following is a sequence at which a restriction enzyme would cut? A) 5'AAGCAA3' B)…
A: "Restriction enzymes" are enzymatic proteins that bacteria generate in order to protect themselves…
Q: With respect to F+ and F- bacterial matings, answer the following questions: (a) How was it…
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Q: What is the relationship between a PRR and a PAMP?
A: Definition: PRRs are specialized receptors expressed by cells of the innate immune system.Function:…
Q: Regarding the cultivation of animal cells in vitro, it is correct to state that: *(Only one…
A: a) Transformed cells differ from normal and established cells in that they have the ability to…
Q: If a mutation occurs in the DNA of cells, the mutation will be passed onto the organism's offspring.…
A: Mutations are the change in the DNA sequence. Mutation can occur in a single base pair or in a long…
Q: You genetically engineer nonadhesive cells to express one variety of cadherins and then mix the…
A: The objective of the question is to understand the behavior of genetically engineered nonadhesive…
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Q: If we dissect the two processes into small steps, can each step be reversible?
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Q: b) Organic matters may interfere with heat treatment of bacterial growth control. ( c)…
A: Please follow step 2 for detailed explanation.
Q: ________ cell culture is similar to that of embryonic stem culture.a) Somatic stem b) Stemc) Gened)…
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Q: 42) If the gene encoding the enzyme rubisco is mutated such that it is non-functional, the process…
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Q: In conjugation of a Hfr cell with an F- cell: a) the container cell becomes F + b) The container…
A: Conjugation is the process by which genetic material, i.e., DNA (deoxyribonucleic acid), transfers…
Which of the following promotes branch migration at higher rates than does Rec-A?
a) Rec-B
b) Rec-C
c) Rec-D
d) Ruv-A and Ruv-B
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- You genetically engineer nonadhesive cells to express one variety of cadherins and then mix the cells in various combinations. You then monitor their interactions. What do you observe?a) The cells adhered preferentially to cells expressing one of the other cadherins.b) The engineered cells adhered preferentially to cells expressing the same cadherins.c) The genetically engineered cells did not stick to any other cells.d) The genetically engineered cells died.e) The genetically engineered cells migrated to a different location.Regarding the cultivation of animal cells in vitro, it is correct to state that: *(Only one statement is correct) a) Transformed cells differ from normal and established cells in that they have the ability to proliferate indefinitely in culture b) Transformed cells derive from isolated and dissected tumors, constituting cell lines that have limited cultivation time c) The genetic instability presented in normal cells makes them preferential models for the production of recombinant proteins d) Cells cultured in adhesion must be periodically expanded, through the use of enzymes such as trypsin e) The metabolism of carbon sources in the culture medium by animal cells generates products such as lactate, which positively influence their productivityWhich of the following is used as a marker in YAC vectors? A) centromere B) X Gal C) auxotrophic markers D) ARS
- We have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…Usually, bacteria only make tryptophan when tryptophan is absent or available in low concentration. However, a particular bacterial mutation makes tryptophan all the time whether or not tryptophan is present. What could explain this phenotype? A) the terminator hairpin is unable to form B) the antiterminator hairpin is unable to form C) trpE is mutated D) trpD is mutated E) trpA is mutatedHow do we know that during transduction bacterial cell-to- cell contact is not essential?
- 62) If you want to inhibit protein synthesis on the transcriptional level you would use: a) Tetracyclin b) Vancomycin c) Macrolides d) Fluroquinolones e) a & c 63) The protein that prevents the formation of off-centered septation in bacteria is: a) FtsZ b) MreB c) MinCD d) Tubulin e) Septin 64) The autoclave pressure must reach at least ________ in order to effectively kill the bacterial spores: a)15 psi b) 15 Joules c) 15 Kilograms d) 15 Lbs e) 15 Kilocalories 65) Incineration is a type of ______ method in controlling bacterial growth: a) moist heat b) dry heat c) chemical d) chemotherapeutic e) genetic.Which of the following is a sequence at which a restriction enzyme would cut? A) 5'AAGCAA3' B) 5'AAGCTT3' C) 5'ACAACA3' D) 5'AGAGAG3' E) 5'AGCCGT3' F) 5'AATTAAC3'42) If the gene encoding the enzyme rubisco is mutated such that it is non-functional, the process that would be affected is the ability to... A) Make ATP B) Harvest photons C) Fix carbon D) Make 02 E) Make NADPH
- 4) Most embryonic stem cells used for research are: Answer: A) totipotent B) pluripotent C) multipotent D) all of the aboveA) Draw a FtsZ mutant cell.B) Draw a MinD mutant cell.C) Draw a MinE mutant cell.An elderly woman took a prescription medicine to help her to sleep; however, she felt restless all night and did not sleep at all. The nurse recognizes that this woman has experienced which type of reaction or effect? a) Allergic reaction b )Idiosyncratic reaction c )Mutagenic effect d )Synergistic effect