Which mode of liquid chromatography would be best for separating the following? The products of a reaction involving common organic compounds. A mixture of o-, m-, p-aminobenzoic acid. The insulin receptor in liver cell extracts `
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Which mode of liquid chromatography would be best for separating the following?
-
- The products of a reaction involving common organic compounds.
- A mixture of o-, m-, p-aminobenzoic acid.
- The insulin receptor in liver cell extracts
`
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- Why do we not need "spitless" injection to measure the toluene content of gasoline using gas chromatography?Chromatography is a separation technique. What is the physical or chemical basis for separation of compounds during thin layer chromatography?Please propose a chromatography-based strategy to purify human insulin proteins that have been expressed in bacterial cells.
- Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.What mode of chromatographic separation (RPLC, NPLC, HILIC, SEC, IEX) is best used for the Separation of uric acids and position isomers of dimethyl uric acids in the urine? What stationary phase and mobile phase (together with elution technique), and other chromatographic conditions needed?1a) Hexane, CH3(CH2)4CH3, is found to have a long retention time when separated from a mixture. A column with a C18 (18-carbon chain) stationary phase and a methanol-water modal phase is used for the separation. Explain why hexane has a long retention time. 1b)You wish to chromatographically seperate a mixtureof the follwing compunds. Which of the two compounds will be most difficult to seperatate from one another? Why?
- 2-pentanone has a retention index of 987 on a poly(ethylene glycol) column (also called Carbowax). (a) Between which two straight-chain hydrocarbons is 2-pentanone eluted? (b) An unretained solute is eluted from a certain column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecane (C11H24) is eluted in 17.22 min. What is the retention time of a compound whose retention index is 1 050?Qno1: why there is a need of chromatography and chromatography thecniqs. B) when and where we should use normal and reverse phase chromatographyIn normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.
- Paper Chromatography and TLC are examples of planar chromatography. True or FalsE?A thin-layer chromatography (TLC) experiement was run using three compunds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with the three compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?Explain how TLC and column chromatography can be used together to ensure the perfect separation of two organic compounds