Which mode of liquid chromatography would be best for separating the following? The products of a reaction involving common organic compounds. A mixture of o-, m-, p-aminobenzoic acid. The insulin receptor in liver cell extracts `
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Which mode of liquid chromatography would be best for separating the following?
-
- The products of a reaction involving common organic compounds.
- A mixture of o-, m-, p-aminobenzoic acid.
- The insulin receptor in liver cell extracts
`
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- Why do we not need "spitless" injection to measure the toluene content of gasoline using gas chromatography?Chromatography is a separation technique. What is the physical or chemical basis for separation of compounds during thin layer chromatography?Please propose a chromatography-based strategy to purify human insulin proteins that have been expressed in bacterial cells.
- Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.1a) Hexane, CH3(CH2)4CH3, is found to have a long retention time when separated from a mixture. A column with a C18 (18-carbon chain) stationary phase and a methanol-water modal phase is used for the separation. Explain why hexane has a long retention time. 1b)You wish to chromatographically seperate a mixtureof the follwing compunds. Which of the two compounds will be most difficult to seperatate from one another? Why?2-pentanone has a retention index of 987 on a poly(ethylene glycol) column (also called Carbowax). (a) Between which two straight-chain hydrocarbons is 2-pentanone eluted? (b) An unretained solute is eluted from a certain column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecane (C11H24) is eluted in 17.22 min. What is the retention time of a compound whose retention index is 1 050?
- In normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.A thin-layer chromatography (TLC) experiement was run using three compunds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with the three compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?Explain how TLC and column chromatography can be used together to ensure the perfect separation of two organic compounds
- 19. What is thin layer chromatography (TLC) LEAST useful for from the list below? A) To determine if crystallization or distillation may be more effective for a separation B) To determine the number of components in a mixture C) To determine the appropriate solvent for column- chromatographic separation D) To monitor the progress of an organic synthesis reaction 14. The solubility of salicylic acid in water is 7.8g/100ml at 100°C and 0.25g/100ml at 25°. Estimate how much water is needed to recrystallize a 19g sample of salicylic acid? A) 150ml B) 250ml C) 300ml D) 400ml1,8-Cineole is a predominant terpene found in cranberries. The concentration of 1,8-cineole in cranberries was determined using gas chromatography. A 5.00 g sample of freeze-dried cranberry powder was added to 10.0 mL water to form a cranberry extract. The extract was filtered. A 1.00 mL aliquot was added to a 25 ml volumetric along with a known amount of internal standard (d-14 cymene) and diluted to volume with water. Standards were spiked with a fixed amount of d-14 cymene as internal standard. The following data was obtained: 1,8-Cineole (ug/mL); 1,8-Cineole (peak area); d-14 cymene (peak area) 10.0; 1260; 1880 15.0; 1865; 1850 20.0; 2520; 1855 50.0; 6280; 1865 Sample; 1976; 1840 What is the concentration of 1,8-cineole in ug per gram of cranberry? O 398 ug/g O 159 ug/g O 674 ug/g O 796 ug/g O 15.9 ug/gPlease answer Questions 5-3, 5-4, and 5-5 (GC Gas Chromatography) 5-2. A sample mixture of ethyl benzoate (bp 212 Celsius) and dodecane (bp 216.2 Celsius) is injected on two GC columns.Column A has DC710 silicone oil as the stationary phase, and column B uses polyethylene glycol as the stationaryphase. Which substance would be certain to elute first from column A and would the same material be expected toelute first from column B? Which column, A or B, would be expected to give the better separation of these two substances? 5-3. Question 5-2 refers to separating a mixture of two high boiling liquids by gas chromatography. These materials havesimilar boiling points. List several GC variables and conditions that would make it easier to separate thesesubstances by gas chromatography. 5-4. Capillary GC columns have better resolution than packed columns even though the enormous surface area provided by the packing material is absent in capillary columns. Why? 5-5. Preparative GC requires…