**Question:** What would be the effect of performing a RT-PCR with the following ingredients: an mRNA template, appropriate primers, dNTPs, heat-stable reverse transcriptase and human DNA polymerase?**Options:**- **a.** The PCR would occur, but with a high mutation rate- **b.** The PCR reaction will not commence- **c.** Non-specific PCR of random templates will occur- **d.** The RNA template would be converted to DNA, but the DNA segment would not be amplified- **e.** PCR would proceed normally

The ingredients required for RT PCR are given below Reverse transcriptase enzyme mRNA template.…

Answered: What would be the effect of performing…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Which of the following natural cellular process does PCR use to amplify a target DNA? A. Gene Expression B. DNA symthesis C. Transcription of DNA D. Translation
Arrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,b
Which of the following chemicals or procedures is not involved in a typical Polymerase Chain Reaction (PCR). Select one: O a. ddNTPs b. O C. O d. Oe. O f. Repeated cycling (typically 32 cycles) for exponential target amplification DNA strand replication/extension Annealing of oligonucleotide primer or primers Thermostable (Taq) DNA polymerase Buffer with required cofactor: Mg 2+ Og. DNA template Oh. High temperature denaturation O i. dNTPs
What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blot
indicate which of the following statements is/are FALSE for qPCR A. The sequence order of steps for a qPCR is 1. Denature 2. Anneal 3. Detect 4. Extend B. qPCR estimates the quantity of template nucleic acid according to the change in fluorescence during the reaction. C. The estimation of nucleic acid quantity is performed at the end point after ~30 -35 PCR cycles D. The absorbance at 260 nm is used in qPCR to estimate the initial amount of DNA in the reaction. E. Intercalating dyes are added to the qPCR to estimate the quantity of dsDNA F. The fluorescent probes used in qPCR are extended by the polymerase
For DNA amplification using PCR to occur, which of the following are needed? A. DNA primers B. thermo stable DNA polymerase C. Helicase D. Choices A and B
Definition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified Organism
Which of the following is true for nested PCR? 1. In the second round of PCR, primers should anneal only to the target site 2. Two primers are used to ensure that one or the other will anneal. 3. Both primers anneal only to the target site, the second round is to get more DNA 4. In the first round of PCR, primers should only anneal to the target site
A region of the genome is amplified by PCR to analyze two closely linked SNPs. PCR products from individual A were labelled with a red-fluorescing dye and those from individual B labelled with a green-fluorescing dye. These PCR products were mixed and hybridized to an oligonucleotide microarray as shown in the figure below. What can we conclude about the data shown? Select all true: a. individual A is heterozygous for the M1 and M2 haplotypes, individual B is heterozygous for M4 and M5 b. individual A is heterozygous for the M1 and M2 haplotypes, individual B is heterozygous for M3 and M6 c. Sanger sequencing traces of these PCR products would show the same double peaks at the same positions for these two individuals d. analyzing this PCR amplified region with conventional Sanger sequencing would be more accurate than the microarray analysis e. individuals A and B could be siblings who share a parent that is homozygous for one of the haplotypes {urgent}
What is the improtance of creating the master mix in the PCR lab? Pick all that apply. a. It makes the reaction run faster. b. It ensures more consistent concentrations of reagents for each tube. c. It helps correct for pipette errors at small volumes. d. It is easier to set up a single tube to add needed components in bulk versus pippetting each sample tube individually.
If you were doing a cloning experiment where a plasmid was treated with PstI but the insert DNA did not have a PstI restriction site, what restriction enzyme(s) would be suitable to treat the DNA to be inserted?
In 1993, Kary Mullis won the Nobel Prize in Chemistry for his invention of the PCR process. Describe the three steps in each cycle of a PCR reaction. Why was the discovery of a thermostable DNA polymerase so important for the development of PCR?

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