PCR is used to: Select one: a. Randomly break large pieces of DNA or plasmids into smaller fragments. O b. Determine if a particular piece of DNA contains a certain gene. C. Amplify a specific segment of DNA. d. Separate DNA fragments based on size. e. Isolate specific pieces of DNA. ess Jump to...

Q: Select all of the following that need to be in a polymerase chain reaction that will successfully…

A: Polymerase chain reaction (PCR) is a molecular technique used by scientists to multiply particular…

Q: Match the terms associated with tne polymerase chain reaction with their correct descriptions.

A: PCR is the polymerase chain reaction in which Denaturation, Annealing and Extension process takes…

Q: A DNA microarray is a slide that is dotted witha. mRNAs from a sample of cells.b. fluorescently…

A: Bio-informatics is the branch of biology that uses computers and statistics to analyse and record…

Q: The image below represents the PCR results after electrophoresis on an agarose gel. One band is…

A: PCR is based on the principle of amplifying DNA through a series of cycling reactions with varying…

Q: a. Explain whether or not DNA polymerase from a mesophilic bacterium could be used successfully in a…

A: A)- In a mesophile optimum condition would allow the microbe to replicate at it maximum amount…

Q: has been assembled by researchers and transplanted into a donor bacterial strain to study never…

A: there are diverse bacterial strains that have an antibiotic resistance system. Using antibiotics may…

Q: The function of a restriction enzyme is to a. prevent the movement of DNA outside the nucleus b.…

A: Restriction enzymes:- these are the proteins and mostly isolated from prokaryotes. These enzymes…

Q: DNA polymerases make errors in matching as DNA is synthesized. These errors can be detected and…

A: DNA replication is the process by which two exact copies of DNA are made from an original DNA…

Q: Match the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts…

A: DNA helicase is used during the DNA replication process where it binds to the double-stranded DNA…

Q: A genetic map provides a. the sequence of the DNA in a genome. b. the relative position of genes…

A: The genome of an organism represents all the genetic information contained within the cells. This…

Q: Which of the following is FALSE about current Sanger dideoxy DNA sequencing procedures?   a. Chain…

A: An oligonucleotide primer is annealed to a single-stranded DNA template and expanded by DNA…

Q: Polymerase Chain Reaction, or PCR, can

A: It is a technique to amplify small segments of DNA.  It makes copies of a specific region of DNA. It…

Q: Gel electrophoresis can be used to separate DNA on the basis of:   A. the probe used.   B. size.…

A: Electrophoresis is a laboratory technique used to separate DNA, RNA or protein molecules. Agarose…

Q: By mistake, you add two primers to your DNA sequencing reaction. The primers anneal on the same…

A: The correct answer is:B. The detector will detect two nucleotides at about 75% of the positions.

Q: Which of the following is NOT true of DNA polymerase?   a.  DNA polymerase builds the new strand…

A: DNA polymerase is a type of enzyme found in all living things. Its primary function is to duplicate…

Q: PCR relies on the use of a pair of primers amplify a portion of DNA Which of the following…

A: The study of the make-up, structure, and interconnections of cellular molecules, that include…

Q: Chromosomal walking is a method of in which                                 researcher begins at a…

A: Chromosomes are long thread-like structures that carry coded genetic information in the form of DNA.…

Q: Which statement is true?   a. There is no danger involved in recombinant DNA research in humans.…

A: Introduction Recombinant DNA technology is a technique that uses specific enzymes for the cutting…

Q: When constructing a recombinant DNA molecule, a marker gene is used to:   a. give the organism a…

A: Answer :- b. Identify whether the transformed organism contains the recombinant DNA

Q: The way PCR amplifies DNA is similar to the doubling in apopulation of growing bacteria—a single DNA…

A: Introduction: PCR or polymerase chain reaction is a laboratory method used to amplify the DNA…

Q: The restriction endonucleases used in recombinant DNA work:   a. are synthesized by bacteria   b.…

A: Specific sequences in the entering DNA are recognized by restriction endonucleases, which then break…

Q: When we discuss PCR and other similar techniques, the term Tm is often used. This refers to a.The…

A: PCR ( Polymerase Chain Reaction) is a technique in which numerous copies of genetic material (…

Q: Place the steps of sanger sequencing in order. A.  A fluorescent laser excites the fragments and…

A: DNA (the DNA that has to be sequenced) is used as the beginning point for DNA synthesis.…

Q: Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger…

A: Q.Explanation:- Introduction:- Sanger sequencing is a method that determines the sequence of a DNA…

Q: Transcriptomes, that is, all the transcripts in a cell, are analyzed using:     a. a type of PCR…

A: Microarrays and sequencing are two methods for studying transcriptomes. Since microarrays offer an…

Q: There are several factors that contribute to high fidelity of DNA replication. Which of the…

A: To preserve genetic integrity and to ensure genetic continuity, DNA that is transferred to daughter…

Q: Which of the following is TRUE with respect to electrophoresis? a. DNA is not attracted to either…

A: Electrophoresis is defined as the motion of dispersed particles relative to a fluid under the…

Q: Certain restriction endonucleases produce cohesive (sticky) ends.  This means that they: a. stick…

A: The primary purpose of restriction endonucleases is to guard against foreign genetic material,…

Q: Some bacteria, through natural selection, have acquired some extremely potent enzymes that destroy…

A: In the perpetual battle between microorganisms, bacteria have evolved remarkable defense mechanisms…

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