Explain the role of each of the two enzymes used in the room temperature -PCR process. Reverse transcriptas Taq DNA polymerase
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Explain the role of each of the two enzymes used in the room temperature -PCR process.
Reverse transcriptas
Taq DNA polymerase
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- Explain why a positive control and negative control are included in PCR experiments. Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis? Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.List and briefly describe the function of 4 proteins used in DNA replication in a E. coli but NOT necessary for PCR.Put the steps of one PCR cycle in the correct order: The PCR reaction mixture is heated to about 70 degrees, which is the optimum temperature for the polymerase to build the new strands of DNA, starting at the 3' end of the primer. The PCR reaction mixture is heated to 95 degrees Celsius, which denatures the double stranded template DNA. The PCR reaction mixture is cooled to about 50-55 degrees, which allows the primers to find their complementary site on the template and "anneal" the
- Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro? In simple terms so, that I can understand. Thank youBriefly explain the rationales of adding chemicals which can affect DNA stability in polymerase chain reaction (PCR).Evaluation of PCR product electrophoresed on 0.8% agarose gel shows non-specific bands. The appropriate modification for the next PCR reaction is to: a) increase the concentration of Taq polymerase b) increase the number of PCR cycles c) decrease the template denaturation temperature d) reduce the concentration of primers
- Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro?Briefly explain the rationales of adding chemicals that can affect DNA stability in a polymerase chain reaction (PCR). Why DNA melting is required in PCR? Briefly explain how PCR can be used to detect DNA mutation.The process of PCR essentially revolves around three phases. Briefly describe these phases and the events that occur in them. Take note the temperature on which these phases take place.
- using the process flow of PCR describe the principle associated with the PCR technique.Briefly describe what happens during each of the phases of PCR (denaturation, annealing, and extension), including when you need each of the major components of the reaction (template, primers, nucleotides, DNA polymerase).Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro? In simple terms so, that I can understand. Thank you