What is the reagent used to detect glucose in the ph and temperature experiments? What is its principle? What is the principle of the spectrophotometer?
Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.
A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…
Q: What is the result for the yellow tube of Methyl red test? What must the pH be in each tube? What is…
A: Methyl red test This test usually aims to differentiate the facultative anaerobes enteric bacteria.…
Q: What is the relationship of concentration and absorbance based on the table and what are the changes…
A: The concentration of a solute in a solution can be determined spectrophotometrically by passing a…
Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?
A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…
Q: Write the materials, reagents, Principle and procedure for the Periodic acid Schiff technique
A: Answer: STAINING = It is the method to colorize the smear or transparent tissue or cells in order to…
Q: What is the range of pocket thermometer?
A: They are lightweight digital thermometers. They have a built in pocket clip. It is constructed using…
Q: What are the underlying physical principles of paper chromatography? What is spectrophotometry, the…
A: Analysis of different compounds quantitatively and qualitatively is an important aspect of research…
Q: Does different kinds or types of tea produces different amount of caffeine content? Why? Why does…
A: Caffeine is categorized as a type of a bitter substance. The caffeine occurs in a number of…
Q: Write shortly about the instrumentation of ATR spectroscopy? Answer should be to the point.
A: ATR(Attenuated Total Reflectance), is the sampling technique used in infrared spectroscopy (IR) to…
Q: What are the advantages and disadvantages of the filter method in the analysis of water samples?
A: Utilising the membrane filter method, test is gone through the layer utilising a filter funnel and…
Q: phenazopyridine
A: Phenazopyridine could be a pain reliever that affects the lower a part of your tract (bladder and…
Q: WHAT ARE THE Steps in spectrophotometer
A: -Spectrophotometer is an optical instrument which is used to measure the concentration of a known…
Q: Suppose you were in a research lab in Romania and the lab is equipped with several…
A: Proteins are biomolecules that play a variety of roles in living systems. They include catalysis,…
Q: What is known as electropherogram ?
A: An analytical procedure that is used for the separation of fragments of DNA or RNA, in terms of…
Q: By the means of gas stoichiometry, how soaps are created?
A: Soap is made up of animal fat or plant oil, as well as caustic soda. It dislodges dirt from surfaces…
Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?
A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…
Q: Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement…
A: An absorption spectrophotometer is defined as a device that is used to calculate the absorbed light…
Q: Using pearson's square, how to attain 30o Brix syrup using 50oBrix and 20o Brix syrup solutions?
A: Here, the given two available syrups are 50°Brix and 20°Brix, that is one of the solutions is higher…
Q: What non-titrimetric method can be used to determine the iodine value of edible oil? Discuss its…
A: Triglycerides are found in fats and oils. Fatty acyl esters join three fatty acids to glycerol to…
Q: What is a Spectrogram
A: Spectrogram is a visual way of viewing the spectrum of frequencies of signal that varies with time.
Q: What are the two principal types of mass spectrometry?
A: Mass spectrometry is used to determine the mass-to-charge ratio of ions and separate them according…
Q: Why are quantitative absorbance measurements made at lambda max?
A: Introduction :- Using spectrum of wavelength , different levels of absorption can be calculated. It…
Q: A student while performing the casein precipitation using isoelectric pH, observed maximum…
A: Casein is the main protein present in milk. It is a phosphoprotein, phosphate groups are attached to…
Q: What are the possible errors that could occur during the titration experiment to determine acetic…
A: Titration is the analytical method which determine the unknown concentration of the chemical…
Q: In a spectrophotometer, what should be the appearance on the graph if the sample is pure? presence…
A: Spectrophometry is a widely used technique in identification and confirmation of chemical…
Q: How do I find the optimal temperature for the iodine and starch experiment?
A: The optimal temperature for a particular enzyme is the temperature at which the enzyme attains…
Q: what does it mean if a pigment (let' say 'yellow') traveled beyond the solvent front in paper…
A: Paper chromatography is the process of separation technique which involved in speraration of…
Q: What is the importance of normalization of data in fluorescence spectroscopy
A: Introduction :- An electromagnetic spectroscopy technique called fluorescence spectroscopy examines…
Q: Why does one see methyl orange as orange in color? What blank would be used to standardize this…
A: 1. Methyl orange is a pH indicator frequently used in titration because of its clear and distinct…
Q: What is the reason for the decrease in the ABS value over time when measuring in the…
A: A spectrophotometer is a device used in an analytical chemistry lab to measures the amount of light…
Q: What is the main application of Atomic absorption spectrophotometer (AAS)?
A: The Atomic Absorption Spectrophotometer (AAS) is primarily used to quantify the presence of several…
Q: What is meant by the term plating efficiency?
A: The cells are cultured over the plate at the particular growth conditions within a particular time…
Q: Explain the color changes observed throughout the peroxide value of oil experiment. Materials: 1.…
A: The unsaturated fat when undergoing oxidation forms peroxide so the peroxide value denotes the…
Q: mportance of pH adjustment in culture media?
A: Culture media is also known as growth media. It is a mixture of nutrients that helps for the growth…
Q: What is the importance of sllid width in fluorescence spectroscopy
A: Fluorescent microscopy is often used to image specific features of small specimens such as microbes.
Q: In the spectrophotometer, why is there a need to measure a 'blank' sample? What will happen to the…
A: A spectrophotometer is one of the most frequent tools used by scientists to determine the presence…
Q: What is the purpose of the respirometer with just rocks (the thermobar)? What conditions would cause…
A: Introduction A respirometer measures the rate of oxygen and/or carbon dioxide exchange in a living…
Q: What is the principle involved in the Dinitro salicylic (DNS) colorimetric method?
A: DNSA refers to 3, 5-dinitrosalicylic acid. It is an aromatic compound. It is widely used in…
Q: What is the meaning of BSC or biological safety cabinet and why is it important and how is it works?
A: Introduction: Biosafety issues refer to the procedures, policies, and principles to be adopted to…
Q: Which of the following is/are source/s of error in performing a spectrophotometric method? Answer…
A: Spectrophotometery It refers to a technique which is helpful in identifying the solute concentration…
Q: What are the advantages of solid media ?
A: Microbial culture is a method of multiplying microbes by allowing them to reproduce in a…
Q: What is the formula to determine the actual size of an organism?
A: “Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: Why is cobalt chloride paper used in the experiments?
A: The indicators are used in the experiment to detect the distinct observable change. Many types of…
Q: Which of the following scientists use this X-ray crystallography?
A: X-ray crystallography is the experimental science determining the atomic and molecular structure of…
Q: What is this instrument? What reading does it provide? What is the tube with only solvent in it…
A: INTRODUCTION Spectrophotometer A spectrophotometer measures the amount of intensity of light a…
Q: What is the stationary and mobile phase of spectrophotometry?
A: Introduction A biotechnique based on the principle of quantitative measurement of light intensity…
Q: What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?
A: Spectroscopy is a procedure utilized for the evaluation, portrayal, and basic investigation of a…
What is the reagent used to detect glucose in the ph and temperature experiments? What is its principle?
What is the principle of the spectrophotometer?
Step by step
Solved in 3 steps
- The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.What is optical density (OD) and how is it measured? How is it related to the concentration of the analyte? And what is a colorimetric assay?The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
- What non-titrimetric method can be used to determine the iodine value of edible oil? Discuss its basic principles and general methods involved.Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement or during the transfer to the spectrophotometer?Using pearson's square, how to attain 30o Brix syrup using 50oBrix and 20o Brix syrup solutions? (please indicate the solution and formula)
- Using the sodium chloride equivalent method, make the following solution isotonic with respect to the mucous lining of the eye (ocular membrane): Tetracycline hydrochloride: 10 g NaCl: x g Sterile distilled water, enough to make 1000 mL Report your answer in grams (to one decimal place).Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.What are the possible errors that could occur during the titration experiment to determine acetic acid content in vinegar? What can be done to prevent these errors? How will you treat your data?