What is the purpose of reference beam in the double-beam spectrophotometer shown in Figure 20-1? Select all the correct answers. a)To correct for changes in source light intensity as a function of time b)to correct for changes in intensity of the light source as a function of wavelength c)to correct of scattering of light off the surface of the sample cuvette d)to correct for absorbance of the solvent e)to correct for changes in detector signal as a function of wavelength
What is the purpose of reference beam in the double-beam spectrophotometer shown in Figure 20-1?
Select all the correct answers.
a)To correct for changes in source light intensity as a function of time
b)to correct for changes in intensity of the light source as a function of wavelength
c)to correct of scattering of light off the surface of the sample cuvette
d)to correct for absorbance of the solvent
e)to correct for changes in detector signal as a function of wavelength
As the name suggests, a double beam spectrophotometer employs two types of beams: reference beam and sampling beam. Reference beak passes through the standard solution while the sampling beam passes through the sample, and their ratio falls on the detector. It compares the intensity between two path systems.
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