What is the Isoelectric point of Y-R-S (pH 7-11) What is the isoelectric point of N-G-L (pH 1-5) Please do the solutions like in the excel table indicated below and follow the pKa value in the table ТАBLE 3.2pk, Values of Common Amino AcidsAcida-COOHa-NH,+RH or RH*Gly2.349.60Ala2.349.69Val2.329.62Leu2.369.68Ile2.369.68Ser2.219.15Thr2.6310.43Met2.289.21Phe1.839.13Тр2.389.39Asn2.028.80Gln2.179.13Pro1.9910.6Asp2.099.823.86*Glu2.199.674.25*His1.829.176.0*10.78CysТyrLys1.718.33*2.209.1110.072.188.9510.53Arg2.179.0412.48*For these amino acids, the R group ionization occurs before the a-NH,* ionization. PeptideК(асоон)G(ANH3)Y(phenol)κ(NH3)Net CHARGECalc. plSteps in approximating pl of a given oligopeptide.1. identify the pk vaiues of ionizable side chains of the given peptide.G-Y-KpK1=2.18pК2-9.60pKR=10.07pKR=10.532. Enumerate the pH leveis 1-14.pH 13. Arrange the pk vaiues in ascending oraer.4. Determine tne cnarge or eacn group at speciTic pH levei:PH 3-1PH 4-1Rule:acidic Rbasic kPH 5-11pk>pHpH 6-11pk

Isoelectric point (PI) is the pH at which the molecule has no net electrical charge. Each amino acid…

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Biochemistry

What is the Isoelectric point of Y-R-S (pH 7-11) What is the isoelectric point of N-G-L (pH 1-5)

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

What is the Isoelectric point of Y-R-S (pH 7-11)

What is the isoelectric point of N-G-L (pH 1-5)

Please do the solutions like in the excel table indicated below and follow the pKa value in the table

ТАBLE 3.2
pk, Values of Common Amino Acids
Acid
a-COOH
a-NH,+
RH or RH*
Gly
2.34
9.60
Ala
2.34
9.69
Val
2.32
9.62
Leu
2.36
9.68
Ile
2.36
9.68
Ser
2.21
9.15
Thr
2.63
10.43
Met
2.28
9.21
Phe
1.83
9.13
Тр
2.38
9.39
Asn
2.02
8.80
Gln
2.17
9.13
Pro
1.99
10.6
Asp
2.09
9.82
3.86*
Glu
2.19
9.67
4.25*
His
1.82
9.17
6.0*
10.78
Cys
Тyr
Lys
1.71
8.33*
2.20
9.11
10.07
2.18
8.95
10.53
Arg
2.17
9.04
12.48
*For these amino acids, the R group ionization occurs before the a-NH,* ionization.

Peptide
К(асоон)
G(ANH3)
Y(phenol)
κ(NH3)
Net CHARGE
Calc. pl
Steps in approximating pl of a given oligopeptide.
1. identify the pk vaiues of ionizable side chains of the given peptide.
G-Y-K
pK1=2.18
pК2-9.60
pKR=10.07
pKR=10.53
2. Enumerate the pH leveis 1-14.
pH 1
3. Arrange the pk vaiues in ascending oraer.
4. Determine tne cnarge or eacn group at speciTic pH levei:
PH 3
-1
PH 4
-1
Rule:
acidic R
basic k
PH 5
-1
1
pk>pH
pH 6
-1
1
pk<pH
-1
PH /
-1
PH 8
-1
5. Find the net charge.
pH 9
-1
9.6
9.6
6. Find the 2 pk values surrounding the pH ievel at wnich the peptide is U-cnarge.
pH 10
-1
pi
PH 11
-1
10.07
-2
10.07
рн 12
-1
-1
-2
рH 13
-1
-1
--
рH 14
9.84
-1
-1
--

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