What is the Isoelectric point of Y-R-S (pH 7-11)  What is the isoelectric point of N-G-L (pH 1-5)

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

What is the Isoelectric point of Y-R-S (pH 7-11) 

What is the isoelectric point of N-G-L (pH 1-5)

 

Please do the solutions like in the excel table indicated below and follow the pKa value in the table 

ТАBLE 3.2
pk, Values of Common Amino Acids
Acid
a-COOH
a-NH,+
RH or RH*
Gly
2.34
9.60
Ala
2.34
9.69
Val
2.32
9.62
Leu
2.36
9.68
Ile
2.36
9.68
Ser
2.21
9.15
Thr
2.63
10.43
Met
2.28
9.21
Phe
1.83
9.13
Тр
2.38
9.39
Asn
2.02
8.80
Gln
2.17
9.13
Pro
1.99
10.6
Asp
2.09
9.82
3.86*
Glu
2.19
9.67
4.25*
His
1.82
9.17
6.0*
10.78
Cys
Тyr
Lys
1.71
8.33*
2.20
9.11
10.07
2.18
8.95
10.53
Arg
2.17
9.04
12.48
*For these amino acids, the R group ionization occurs before the a-NH,* ionization.
Transcribed Image Text:ТАBLE 3.2 pk, Values of Common Amino Acids Acid a-COOH a-NH,+ RH or RH* Gly 2.34 9.60 Ala 2.34 9.69 Val 2.32 9.62 Leu 2.36 9.68 Ile 2.36 9.68 Ser 2.21 9.15 Thr 2.63 10.43 Met 2.28 9.21 Phe 1.83 9.13 Тр 2.38 9.39 Asn 2.02 8.80 Gln 2.17 9.13 Pro 1.99 10.6 Asp 2.09 9.82 3.86* Glu 2.19 9.67 4.25* His 1.82 9.17 6.0* 10.78 Cys Тyr Lys 1.71 8.33* 2.20 9.11 10.07 2.18 8.95 10.53 Arg 2.17 9.04 12.48 *For these amino acids, the R group ionization occurs before the a-NH,* ionization.
Peptide
К(асоон)
G(ANH3)
Y(phenol)
κ(NH3)
Net CHARGE
Calc. pl
Steps in approximating pl of a given oligopeptide.
1. identify the pk vaiues of ionizable side chains of the given peptide.
G-Y-K
pK1=2.18
pК2-9.60
pKR=10.07
pKR=10.53
2. Enumerate the pH leveis 1-14.
pH 1
3. Arrange the pk vaiues in ascending oraer.
4. Determine tne cnarge or eacn group at speciTic pH levei:
PH 3
-1
PH 4
-1
Rule:
acidic R
basic k
PH 5
-1
1
pk>pH
pH 6
-1
1
pk<pH
-1
PH /
-1
PH 8
-1
5. Find the net charge.
pH 9
-1
9.6
9.6
6. Find the 2 pk values surrounding the pH ievel at wnich the peptide is U-cnarge.
pH 10
-1
pi
PH 11
-1
10.07
-2
10.07
рн 12
-1
-1
-2
рH 13
-1
-1
--
рH 14
9.84
-1
-1
--
Transcribed Image Text:Peptide К(асоон) G(ANH3) Y(phenol) κ(NH3) Net CHARGE Calc. pl Steps in approximating pl of a given oligopeptide. 1. identify the pk vaiues of ionizable side chains of the given peptide. G-Y-K pK1=2.18 pК2-9.60 pKR=10.07 pKR=10.53 2. Enumerate the pH leveis 1-14. pH 1 3. Arrange the pk vaiues in ascending oraer. 4. Determine tne cnarge or eacn group at speciTic pH levei: PH 3 -1 PH 4 -1 Rule: acidic R basic k PH 5 -1 1 pk>pH pH 6 -1 1 pk<pH -1 PH / -1 PH 8 -1 5. Find the net charge. pH 9 -1 9.6 9.6 6. Find the 2 pk values surrounding the pH ievel at wnich the peptide is U-cnarge. pH 10 -1 pi PH 11 -1 10.07 -2 10.07 рн 12 -1 -1 -2 рH 13 -1 -1 -- рH 14 9.84 -1 -1 --
Expert Solution
steps

Step by step

Solved in 3 steps with 2 images

Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON