What is the Isoelectric point of Y-R-S (pH 7-11) What is the isoelectric point of N-G-L (pH 1-5)
What is the Isoelectric point of Y-R-S (pH 7-11) What is the isoelectric point of N-G-L (pH 1-5)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
What is the Isoelectric point of Y-R-S (pH 7-11)
What is the isoelectric point of N-G-L (pH 1-5)
Please do the solutions like in the excel table indicated below and follow the pKa value in the table

Transcribed Image Text:ТАBLE 3.2
pk, Values of Common Amino Acids
Acid
a-COOH
a-NH,+
RH or RH*
Gly
2.34
9.60
Ala
2.34
9.69
Val
2.32
9.62
Leu
2.36
9.68
Ile
2.36
9.68
Ser
2.21
9.15
Thr
2.63
10.43
Met
2.28
9.21
Phe
1.83
9.13
Тр
2.38
9.39
Asn
2.02
8.80
Gln
2.17
9.13
Pro
1.99
10.6
Asp
2.09
9.82
3.86*
Glu
2.19
9.67
4.25*
His
1.82
9.17
6.0*
10.78
Cys
Тyr
Lys
1.71
8.33*
2.20
9.11
10.07
2.18
8.95
10.53
Arg
2.17
9.04
12.48
*For these amino acids, the R group ionization occurs before the a-NH,* ionization.

Transcribed Image Text:Peptide
К(асоон)
G(ANH3)
Y(phenol)
κ(NH3)
Net CHARGE
Calc. pl
Steps in approximating pl of a given oligopeptide.
1. identify the pk vaiues of ionizable side chains of the given peptide.
G-Y-K
pK1=2.18
pК2-9.60
pKR=10.07
pKR=10.53
2. Enumerate the pH leveis 1-14.
pH 1
3. Arrange the pk vaiues in ascending oraer.
4. Determine tne cnarge or eacn group at speciTic pH levei:
PH 3
-1
PH 4
-1
Rule:
acidic R
basic k
PH 5
-1
1
pk>pH
pH 6
-1
1
pk<pH
-1
PH /
-1
PH 8
-1
5. Find the net charge.
pH 9
-1
9.6
9.6
6. Find the 2 pk values surrounding the pH ievel at wnich the peptide is U-cnarge.
pH 10
-1
pi
PH 11
-1
10.07
-2
10.07
рн 12
-1
-1
-2
рH 13
-1
-1
--
рH 14
9.84
-1
-1
--
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