What is a major drawback of performing genome editing with site-specific endonucleases over RNA-guided endonucleases? Question 25 options: difficulty in transformation Necessity of protein cargo to facilitate the editing the need to genetically engineer a new endonuclease for each target sequence. Specificity is not achieved
Question 36
Using Sanger sequencing, starting from the sequencing primer, what is the sequence of the DNA sample ?
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G T A C C C G A A A T C A G G A |
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A G G A C T A A A G C C C A T G |
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G T A C C C G A A T T C A G G A |
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A G C A C T A A A G C C C A T G |
Question 25
What is a major drawback of performing genome editing with site-specific endonucleases over RNA-guided endonucleases?
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difficulty in transformation |
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Necessity of protein cargo to facilitate the editing |
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the need to genetically engineer a new endonuclease for each target sequence. |
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Specificity is not achieved |
Question 23
What is not true for Sequence tagged site (STS) markers:
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cannot be mapped by fluorescence in situ hybridization (FISH) |
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subset of STS markers are known as expressed sequence tag (EST) markers |
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can readily be screened by a PCR assay |
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short DNA sequences that occur at a unique location in the genome |
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