In order for PCR (polymerase chain reaction) to be possible, we need to add reaction: O a DNA polymerase that can work at high temperature, e.g., 72C. O MRNA

Q: You are using a spectrophotometer to determine the concentration of DNA in a PCR product, but your…

A: The concentration of DNA and RNA should be determined by measuring the absorbance at 260 nm (A260)…

Q: Which of the following plates could represent the results of the transformation experiment with PGLO…

A: INTRODUCTION Transformation is a genetic exchange mechanism by which “naked” DNA is taken up by…

Q: PCR is a very useful method in biotechnology because it does not require the 6 or more different key…

A: PCR is a polymerase chain reaction- it ia process of replication of DNA in laboratory. PCR can…

Q: According to Chargaff's observations of nucleotide composition of DNA samples OA % of (G + C) + % of…

A: Chargaff's rule has been stated for the DNA, which states that the ratio of purine and pyrimidine…

Q: During a PCR reaction, the step in the cycle where dNTPs are added to the primer 3’ ends to fill in…

A: Kary Mullis created the groundbreaking technique known as PCR (Polymerase Chain Reaction) in the…

Q: When molecular biologists carry out a PCR, they have a range of different DNA polymerase enzymes on…

A: Polymerase chain reaction (PCR) is a frequently used approach for rapidly producing millions or even…

Q: You want to set up 50 µl total volume of a PCR reaction. You have a microfuge tube of Taq polymerase…

A:

Q: In a PCR reaction to amply a particular gene, 120 copy of the gene were initially used as DNA…

A: Here, option A is the right answer. You may use the following formula to calculate the number of…

Q: If you wanted PCR to be successful but only had access to a thermolabile DNA polymerase, what would…

A: PCR - Polymerase chain reaction, is a process where DNA copies are amplified by a cyclic chain…

Q: molecules continues to double every temperature cycle (referred to as a PCR cycle). (a) Suppose a…

A: Note: We can only answer one question at a time so please resubmit other questions one by one.…

Q: Primers are used in both PCR and in DNA sequencing. What is the purpose of the primers? O to…

A: The laboratory procedure known as PCR (polymerase chain reaction) is used to amplify (produce…

Q: If the primer that you design anneals to two different regions of the DNA to be sequenced, the…

A: A primer is a short sequence of nucleic acids that serves as a starting point for DNA synthesis.…

Q: If you were to set up a PCR reaction (in vitro DNA synthesis) with a DNA template, primers,DNA…

A: PCR is an in vitro technique for the DNA synthesis. It is used to generate the multiple copies of…

Q: 2) A scientist wants to increase the amount of sample of a DNA sequence of 100 bps. Calculate the…

A: Given that molar mass of average DNA base pair is 600g/mol. That means 600g of base pairs contain…

Q: In PCR amplification reaction, 5 mM EDTA was erroneously added to your final reaction, and you did…

A: Please follow step 2 for detailed explanation.

Q: To relax a supercoiled DNA, you may use, Select one: O a. DNA topoisomerase I O b. DNA helicase O c.…

A: Ans:- (a) DNA topoisomerase I

Q: How do you know if it works? If you have more than one band in a lane, why what would be the reason

A: PCR is a polymerase chain reaction and it is used to amplify the segment of DNA/Nucleic acid.

Q: The DNA polymerase used in PCR is taken from Thermus aquaticus, a bacterium that thrives in the…

A: The PCR or polymerase chain reaction is the process that produce multiple copy number from a…

Q: A segment of DNA has the following sequence:TTGGATGCTGAACCTACGACA. What would the sequence be…

A: DNA has 2 types of base pairs purines and pyrimidines. Purines consist of adenine and guanine while…

Q: Running a PCR Why is a Hotstart necessary and how is it achieved? Why are the three temperatures…

A: Answer: 1)In order to avoid non-specific DNA amplification, hotstart is required in PCR. The…

Q: Why might a PCR reaction not work for a 3500bp region you want to amplify when the reaction worked…

A: In molecular biology, the polymerase chain reaction is a method that is employed to amplify a target…

Q: What is expected theoretical number of copies of DNA molecules after 28 cycles in a PCR experiment?…

A: INTRODUCTION: DNA : Its fullform is Deoxyribo nucleic acid and It has a double stranded helix…

Q: if You have been transported to a DNA Lab by a mad scientist.    The scientist hands you a gel…

A: DNA is one of the forms of nucleic acid and can be observed as a genetic material in most of the…

Q: Transformation can be described as:

A: Prokaryotes are unicellular organisms that reproduce in two ways namely- Asexual reproduction and…

Q: In a PCR-based crime scene investigation, similar to the one presented in the lab module with…

A: The RAPD or rapid amplification of polymorphic DNA is used to detect the DNA that is present in the…

Q: Suppose that you want to clone a gene that has never been analyzed before by DNA sequencing. Which…

A: PCR is used to amplify the target DNA sequence and it makes multiple copies of a target DNA sequence…

Q: During gel electrophoresis, in what direction (toward which electrode) does DNA move toward?…

A: There are different molecular biology tools and techniques used to analyze and quantify…

Q: Write the nucleotide sequence, including the primer, in the 3'to 5' direction. 3'- -5'

A: The current iteration of the dideoxy chain termination sequencing technique is dye terminator…

Q: Gel electrophoresis drives DNA along an electrical current from a negative electrode to a positive…

A: Gel electrophoresis is a technique used to separate and analyze macromolecules such as RNA…

Q: Explain why DNA ladders are usually included during gel electrophoresis.

A: BASIC INFORMATION  GEL ELECTROPHORESIS  It is a process in which the DNA fragments are separated…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.