What information do we get if we calculate the ratio of the Absorbance value at 260 nm to the Absorbance value at 280 nm?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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2247
22.00
22.00
20.00
18.00
16.00-
14.00
Sample type
HINA 40
Sampie D
12.00-
10.00
9 8.00-
6.00-
4.00
2.00
Samplee
20 Abs 224
4-260 10 mm path (23.055
A-280 10 mm path (11.20
200/280 2.00
200/230 1
0.00
-2.55
220 230 240 250 260 270 282 290 300 310 320 330 340 350
nglu s222
Wavelenath nm
We have extracted nucleic acids from tissue. A spectrophotometer was used to
analyze the samples.
What information do we get if we calculate the ratio of the Absorbance value at
260 nm to the Absorbance value at 280 nm?
10 mm Absorbance
Transcribed Image Text:2247 22.00 22.00 20.00 18.00 16.00- 14.00 Sample type HINA 40 Sampie D 12.00- 10.00 9 8.00- 6.00- 4.00 2.00 Samplee 20 Abs 224 4-260 10 mm path (23.055 A-280 10 mm path (11.20 200/280 2.00 200/230 1 0.00 -2.55 220 230 240 250 260 270 282 290 300 310 320 330 340 350 nglu s222 Wavelenath nm We have extracted nucleic acids from tissue. A spectrophotometer was used to analyze the samples. What information do we get if we calculate the ratio of the Absorbance value at 260 nm to the Absorbance value at 280 nm? 10 mm Absorbance
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