What are the numbers of theoretical plates for the two analytes, respectively? Suppose an unretained species has an elution time of 1 min, calculate the selectivity factor for acetylsalicylic acid and caffeine.
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What are the numbers of theoretical plates for the two analytes, respectively?
Suppose an unretained species has an elution time of 1 min, calculate the selectivity factor for acetylsalicylic acid and caffeine.
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- 5. Which term is INCORRECTLY defined? a. linear range - the range over which the response of an analytical method is proportional to analyte concentration b. dynamic range concentration range over which there is a measurable response to analyte, though the response is not linear c. blank solution - a solution containing all reagents and solvents used in the analysis to which no analyte is deliberately added d. standard solutions - solutions for which the standard deviation is known - 6. True or False: Internal standards correct random error caused by run-to-run variation. a. True b. False▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? A) methanol B) Ethanol chu C) IPA▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? ▪ A) methanol B) Ethanol ▪ C) IPA
- [1] In paper chromatography, why do some ions move only a short distance from the point of application while others move much more?A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 200.8 s with a baseline peak width, we, of 16.1 s. The retention time for aspartame, ta, was 258.7 s with a baseline peak width, wa, of 20.6 s. The retention time for the unretained solvent methanol was 44.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = Calculate the resolution, R, for this separation using the widths of the peaks. R = 88.2 R₂5N = 3.16 Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5. 3.533 Incorrect μmA student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, tc, was found to be 209.6 s with a baseline peak width, w., of 14.4 s. The retention time for aspartame, ta, was 260.0 s with a baseline peak width, wa, of 21.2 s. The retention time for the unretained solvent methanol was 49.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = um %3D Calculate the resolution, R, for this separation using the widths of the peaks. R = Calculate the resolution if the number of theoretical plates were to increase by a factor of 2. R2N =
- Calculate the concentration of unknown red dye solution solution is not =12.9,134,12.55,0.34After 10.0 mL of an aqueous solution containing 1.235 mg of a drug extracted with 5.0 mL of toluene the aqueous layer was found to contain 0.346 mg of the compound. (a) Calculate the distribution coefficient for the drug between the two solvents. (b) How many 10.0-mL extractions would be required to recover 99.0% of the drug from 20.0 mL of water? What volume of toluene would be used?A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.
- In a volumetric determination of an analyte A, the data obtained and their standard deviations are as follows: Initial buret reading 0.19 mL 0.02 mL Final buret reading 7.86 mL 0.03 mL Sample mass 63.0 mg 0.2 mg From the data, find the coefficient of variation of the final result for the %A that is obtained by using the equation that follows and assuming there is no uncertainty in the equivalent mass. %A= titrant volume x equivalent mass x 100 %/ sample mass Coefficient of variation = Submit Answer % Try Another Version 10 item attempts remainingin AUDIO tutorial is available for this problem. Species of arsenic found in drinking water include AsO33- (arsenite), AsO43- (arsenate), (CH3)2ASO2° (dimethylarsinate), and CH3)AsO3²- (methylarsonate). Pure water containing no arsenic was spiked with 0.40 µg arsenate/L. Seven replicate determinations gave 0.37, 0.38, 0.35, 0.39, 0.39, 0.35, and 0.38 µg/L. (a) Find the mean. 4.0 Hg/L Find the standard deviation. 4.0 ug/L Find the mean percent recovery of the spike. 4.0 % (b) Find the concentration detection limit. 4.0 µg/LDescribe one alternative column other than a C18 for caffeine detection in terms of stationary phase
