We have three inhibitor and same enzyme were incubated with 5 mM of substrate and 2 mM of inhibitor.I1,competitive inhibitor, I2,uncompetitive inhibitor, I3, non-competitive inhibitor.Assuming that Vmax = 80 mM s-1 , Km = 8 mM, and KI of all three inhibitors was 10 mM, which of the inhibitors was the most efficient on V0? Explain your answer

Q: If we made a Lineweaver-Burk plot of an irreversible inhibitor, which type of reversible inhibition…

A: In a Lineweaver-Burk plot for an irreversible inhibition, the most likely resemble inhibitor has to…

Q: a) Determine kcat (in units of sec-1) for a particular enzyme, given the following information: Vo =…

A: The Michaelis Menten reaction rate is given as, V0=K2S∘E∘S∘+KM V0=VmaxE∘S∘+KM where, Vmax=K2S∘…

Q: † 14.) A reversible inhibitor that is unable to bind to the ES complex A) always binds in the active…

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Q: What is the Vmax of an enzyme at 1 µM with an activation free energy (AG#) of 55000 J/mol at 298K?…

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Q: Consider the same enzyme catalyzed reaction with KM 25 mM, Vm-100 mM/s, and [S]20 mM. A…

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Q: A research group discovers a neW version oI happyasee, which they nappyase", that catalyzes the…

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Q: 2. Using the following data, determine the Vmax and Km of the enzyme catalyzed reaction: [S] (M) 2.5…

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Q: Calculate the specificity constant for an enzyme-catalyzed reaction if the total enzyme…

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Q: An enzyme catalyzed the reaction: S -> P has a AG°' value of +4.8 kJ/mol. If you start with a…

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Q: Estimate KI for a competitive inhibitor when [I] = 5 mM gives an apparent value of KM that is three…

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Q: Classify each phrase as describing a competitive inhibitor, uncompetitive inhibitor, or mixed (mixed…

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Q: Q1: the following table gives the results of enzyme-catalyzed reaction. The enzyme concentration [E]…

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Q: The following results were obtained for an enzyme-catalyzed reaction Substrate concentration (mmol…

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Q: The Ksp expression for magnesium phosphate is Mg3(PO4)2(S) 3 Mg2+ (aq) + 2 PO4³-(aq). True False

A:

Q: Based on the experimental results shown in the table, Identify whether the inhibitor acting on this…

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Q: You performed a kinetic study of the enzyme lipase and you obtained the following double reciprocal…

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Q: 1- Calculate Km and Vmax for both experiments using Michaelis-Menten equation. [S] vs Vo 2-…

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Q: Assume that an enzyme has the following kinetic constants: Vmax = 50 umol substrate transformed per…

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Q: Kinetics data were gathered for an enzyme-catalyzed reaction under identical conditions both with…

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Q: 5. An enzyme catalyzes the reaction M = concentration of 1 nM, and the Vmax is 2 µM s-1. The Km for…

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Q: Enzyme X can act on either substrate A or B; the Km on A Vmax on B.Sketch 1 plot for 1/v vs. 1/[S]…

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Q: Q7. Kinetics were analysed for an enzyme in the absence and presence of inhibitor. In the absence of…

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Q: Suppose that you have an enzyme concentration at the molarity calculated in question 2 and the…

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Q: Following data were obtained for an enzyme in the absence and presence of an inhibitor. What are the…

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Q: What is the relative activite and the degree of inhibition caused by a competitive inhibitor when…

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Q: Is the inhibitor competitive, uncompetitive or non competitive and why?

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Q: What is the instantaneous reaction rate for an enzyme system? k = 0.087 1/h; enzyme concentration =…

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Q: Please study the table. Given the data collected for an enzyme-catalyzed reaction, what is the…

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Q: An enzyme catalyzes the reaction M ⇌ N. The enzyme is present at a concentration of 1 nM, and the…

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Q: Trypsin is a serine protease that has a KM of 15 mM. At an initial substrate concentration of 250 µM…

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Q: Relation between Reaction Velocity and Substrate Concentration: MichaelisMenten Equation: At what…

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Q: :The following data were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics…

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.