Q1: the following table gives the results of enzyme-catalyzed reaction. The enzyme concentration [E] = 0.00012 mmol/L Use the information of substrate concentration [S] and rate Vo for both experiments, with inhibitor and without inhibitor to answer the following questions (NOTE: [E] = 0.00012 mmol/L) [S], mmol/L 0.710 0.400 0.310 0.098 0.066 0.040 Experiment 1 without inhibitor VO, mmol/L-min 0.200 0.180 0.160 0.120 0.100 0.070 Experiment 2 with inhibitor [1] = 0.033 mmol/L VO, mmol/L-min 0.180 0.150 0.110 0.070 0.050 0.040 1- Calculate Km and Vmax for both experiments using Michaelis-Menten equation. [S] vs Vo 2- Calculate Km and Vmax for both experiments using Lineweaver-Burk plot, 1/[S] vs 1/vo 3- Calculate Kcat for the enzyme (without inhibition) 4- Based on the values of Km and Vmax, with inhibitor and without inhibitor, determine the type of the Inhibition (competitive, non-competitive or uncompetitive) Note:- show all your work to answer the above questions. Upload your answers as one file then submit it before the due date (03-01-2024)

Q1: the following table gives the results of enzyme-catalyzed reaction. The enzyme concentration [E] = 0.00012 mmol/L Use the information of substrate concentration [S] and rate Vo for both experiments, with inhibitor and without inhibitor to answer the following questions (NOTE: [E] = 0.00012 mmol/L) [S], mmol/L 0.710 0.400 0.310 0.098 0.066 0.040 Experiment 1 without inhibitor VO, mmol/L-min 0.200 0.180 0.160 0.120 0.100 0.070 Experiment 2 with inhibitor [1] = 0.033 mmol/L VO, mmol/L-min 0.180 0.150 0.110 0.070 0.050 0.040 1- Calculate Km and Vmax for both experiments using Michaelis-Menten equation. [S] vs Vo 2- Calculate Km and Vmax for both experiments using Lineweaver-Burk plot, 1/[S] vs 1/vo 3- Calculate Kcat for the enzyme (without inhibition) 4- Based on the values of Km and Vmax, with inhibitor and without inhibitor, determine the type of the Inhibition (competitive, non-competitive or uncompetitive) Note:- show all your work to answer the above questions. Upload your answers as one file then submit it before the due date (03-01-2024)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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