Visible Growth in Tubes Growth in Petri Dishes 0 μg/ml 2μg/ml 0 + 1 2 + + + + 3 + 5 6 0000 a. Determine the minimum inhibitory concentration (MIC). b. Determine the minimum bactericidal concentration (MBC). c. Propose a method to reduce MIC and MBC. 4 7 number 1, and After addition of The figure given above summarizes an experiment carried out to test the efficacy of an antimicrobial substance. In the tube number 0, no antimicrobial substance was added into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube the amount of antimicrobial was doubled for each consecutive tube. antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In addition to observation of visible growth, 1 ml of sample from each tube was collected and plated into petri dishes and incubated for additional overnight. Similarly, the growth status in petri dishes was as (+) and (-). According to given information;

Visible Growth in Tubes Growth in Petri Dishes 0 μg/ml 2μg/ml 0 + 1 2 + + + + 3 + 5 6 0000 a. Determine the minimum inhibitory concentration (MIC). b. Determine the minimum bactericidal concentration (MBC). c. Propose a method to reduce MIC and MBC. 4 7 number 1, and After addition of The figure given above summarizes an experiment carried out to test the efficacy of an antimicrobial substance. In the tube number 0, no antimicrobial substance was added into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube the amount of antimicrobial was doubled for each consecutive tube. antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In addition to observation of visible growth, 1 ml of sample from each tube was collected and plated into petri dishes and incubated for additional overnight. Similarly, the growth status in petri dishes was as (+) and (-). According to given information;

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

Reviewing drug characteristics, choose an antimicrobial for each of the following situations (explain your choice): a. for an adult patient suffering from Mycoplasma pneumonia b. for a child with bacterial meningitis (drug must enter into cerebrospinal fl uid) c. for a patient who has chlamydiosis and an allergy to azithromycin d. for treating a child with Rocky Mountain spotted fever e. for PPNG drug-resistant gonorrhea
E-strip testing can be used to determine a drug MIC against an organism. Which ONE of the following is correct? Select one: A. The zone of inhibition is inversely proportional to the activity of the drug B. The E-strip result guides to the maximum drug concentration that can be used to clear an infection C. The E-strip is impregnated with a fixed concentration of drug D. The E-strip is used broadly clinically to determine if an organism is resistant to a drug E. The MIC is read where bacterial growth touches the E-strip
(1) How did u measure the zone of inhibition (2) Which unit of measurement was used (3) For two organisms,choose two antimicrobial and explain whether the organism was susceptible, intermediate or resistant. Please make the answer as short as possible .Thanks
Think about minimal inhibitory concentration (MIC) assays. A set of tubes, all containing the same amount of bacterial cells, have decreasing amount of antibiotic X added to them. Tube 1 had 0mg/ml, Tube 2 has 100mg/ml, tube 3 has 50mg/ml, 4=25, 5=12.5, 6=6.25, and tube 7 has 3.125. If bacteria grew in tubes 1, 4, 5, 6, 7, what is the MIC of this antibiotic for this bacteria? Select one: a.None of the Above b.3.125 mg/ml c.25 mg/ml d.0 mg/ml e.50 mg/ml
Mechanism of action of electron transport inhibitors. Antimycin A.
Antibiotic sensitivity testing: The Kirby-Bauer. 1) What do the following terms mean? a. PPNG b. MRSA 2) How does the MIC test differ from the Kirby-Bauer test? 3) Were all of the conditions of a standardized Kirby-Bauer test met as you performed this assay? If not, which were not? 4) What is the significance of colonies that develop within otherwise clear zones of inhibition? If the laboratory report for one of your patients indicated colonies within the zone, what concerns would you have for your patient?
Antibiotic Zone of Inhibition A 15 mm B O mm 7 mm 15 mm a. Which antibiotic was most effective against the bacteria being tested? b. Which antibiotic would you recommend for treating a disease caused by this bacterium? c. Was antibiotic A bactericidal or bacteriostatic? How can you tell?
Lophotoxin identify the species which releases the toxin (if it is man-made then this will be all that is required for this part) identify the step disrupted in the neuromuscular junction pathway Provide any consequences of this disruption. Does the toxin have any applications in biomedicine as a painkiller, disease treatment or analgesic? Provide your source in APA format for each. If this is missing no credit will be awarded.
Based on the growth curve, which ONE of the following best describes what would happen if sample of culture in the presence of drug B is inoculated onto drug-free growth medium? Select one: A. Growth present, but cell count increased by at least 99.9% of the original culture B. No growth after 24 hrs C. Growth at 24 hrs D. Not possible to predict because the culture could either be alive or dead E. Growth present, but cell count reduced by at least 99.9% of the original culture
Your nasal sample showed colonies with a clear halo around them on blood agar. What can you determine based on that observation? a. The organism is a mannitol fermenter b. The organism is able to utilize starch c. The organism is reducing the hemoglobin and damaging the erythrocytes d. The organism is beta-hemolytic e. The organism is unable to use red blood cells as a nutrient source
HCl is involved in a. All of the above b. antimicrobial activity. | c. protein denaturation. O d.D cell activation. e. pepsin activation.
5. LC50 of drug X acting on the fibroblast cells for 24hrs is going to be determined by MTT assay. a) 5ml of 1 x 10$ cells/ ml cell suspension is required to be prepared for seeding on the cell culture plate for the assay. How that cell suspension is prepared if the following data is given on the cell stock suspension? (6%) Hemocytometer No. of bright dots 1st large square 52 2nd large square 48 3rd large square 51 4th large square Dilution factor: 5 49