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b. Which variant would be most effective at measuring changes in the concentration of TNB in regions with and without land mines (i.e., where [TNB] could range from 0-2 mM). Briefly explain your answer.

b. Which variant would be most effective at measuring changes in the concentration of TNB in regions with and without land mines (i.e., where [TNB] could range from 0-2 mM). Briefly explain your answer.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Answer part b
engineer the maltose binding protein (MBP) to bind 1, 3, 5 Trinitrobenzene (TNB) - a molecule that is even more explosive than TNT! Instead of its natural ligand, maltose, you hope to develop your mutant into a biosensor that can be used to detect the presence of land mines through identification of TNB in the soil. NO₂ CH₂OH CH₂OH OH OH å ove O₂N NO₂ OH OH Maltose TNB OH After the laborious process of mutating the active site of MBP to bind TNB, you identify four variants of MBP that bind to TNB with the following affinities: TNB-binding MBP variant A B C D KD (μm) 850 9,000 0.4 30 The maximal concentration of TNB that leeches out of land mines into the surrounding soil reaches only 2 mM (milli-molar). Your advisor asks you to use the Ko values you measured to determine which of the variants would be the best candidate for the following purposes. Assume that the fraction of TNB-bound MBP correlates linearly with a signal in your biosensor device from 0 (completely unbound) to 1 (fully bound).
Transcribed Image Text:engineer the maltose binding protein (MBP) to bind 1, 3, 5 Trinitrobenzene (TNB) - a molecule that is even more explosive than TNT! Instead of its natural ligand, maltose, you hope to develop your mutant into a biosensor that can be used to detect the presence of land mines through identification of TNB in the soil. NO₂ CH₂OH CH₂OH OH OH å ove O₂N NO₂ OH OH Maltose TNB OH After the laborious process of mutating the active site of MBP to bind TNB, you identify four variants of MBP that bind to TNB with the following affinities: TNB-binding MBP variant A B C D KD (μm) 850 9,000 0.4 30 The maximal concentration of TNB that leeches out of land mines into the surrounding soil reaches only 2 mM (milli-molar). Your advisor asks you to use the Ko values you measured to determine which of the variants would be the best candidate for the following purposes. Assume that the fraction of TNB-bound MBP correlates linearly with a signal in your biosensor device from 0 (completely unbound) to 1 (fully bound).
b. Which variant would be most effective at measuring changes in the concentration of TNB in regions with and without land mines (i.e., where [TNB] could range from 0-2 mm). Briefly explain your answer.
Transcribed Image Text:b. Which variant would be most effective at measuring changes in the concentration of TNB in regions with and without land mines (i.e., where [TNB] could range from 0-2 mm). Briefly explain your answer.
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