Using my chart can you help me with this question. The tests we used were qualitative; they allowed us to determine if a biological molecule was present or absent. In contrast, what type of information would a quantitative test provide?

Q: Dr. Riggs took 1ml of whole mouse blood and added it to 10ml of PBS. You next take 0.5 mL of Dr.…

A: The first step is to calculate the average number of cells counted in the hemacytometer. The counts…

Q: You have a suspension of cells at a concentration of 5 x 106 cells/mL in culture media. The protocol…

A: Serial dilution process is routinely used to calculate the concentration of unknown solution. The…

Q: SDS-PAGE. FRAP. thin-layer chromatography. Western blotting. O freeze-fracture microscopy.

A: Protein purification is important for the characterization of the function, structure and…

Q: To determine whether proteins interact with each other which two techniques might you use 1. Yeast…

A: Protein-protein interactions (PPIs) are highly-specific physical contacts that occur between two or…

Q: You are looking at a specimen on low power and you cannot see details that your instructor asked you…

A: If I am looking at a specimen at low power and can't see details that my instructor asked me to…

Q: How to write the methodology? I have a practical of centrifugation, I need to do a lab report, but I…

A: The methods section of a research paper outlines the steps taken to investigate a research problem…

Q: A colleague performs a smear prep from a colony on an agar plate. Without staining it, he looks at…

A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…

Q: You are given an unknown bacterial culture and you want to determine if it is resistant to…

A: Antibiotics are chemicals that fight bacterial infections in humans and animals. They work by…

Q: Doing Benedict’s test, how do positive and negative controls help you interpret your test results…

A: A chemical test called Benedict's test can be performed to determine if an analyte contains reducing…

Q: In size exclusion chromatography, which component will elute last in the set up?   Group of answer…

A: Size exclusion chromatography is also known as gel filtration chromatography (GFC) or gel permeation…

Q: Figure 5: MCF-7 cells were incubated for 24 h with the drug dilution buffer (control) or various…

A: Microtubules are made up of protein tubulins that can undergo quick growth or dissolution by…

Q: You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and…

A: A colony-forming unit (cfu) is a unit used in microbiology to estimate the number of viable bacteria…

Q: Complete the following questions for your gram negative rod unknown organism (a) What test did you…

A: The gram-negative rod group is a diverse and extensive category of bacteria characterized by their…

Q: During electrophoresis molecules are separated by three characteristics.  What are they?

A: Electrophoresis is a technique commonly used in the lab to separate charged molecules, like DNA,…

Q: Explain the role of scrubbing liquid, salt, meat tenderizer, and alcohol in the experiment of…

A: Nucleic acid extraction is the method to isolate the nucleic acid from the cell. It involves three…

Q: You got an opportunity to join a professor lab who is working in-vivo model and specifically looking…

A: Mitochondria are called the power house of the cells as it is the site where the maximum amount of…

Q: When viewed under a microscope, bacteria appear as red rods after they have been stained. Explain a…

A: Introduction Bacteria Are Minute, Single-celled Organisms That Can Be Found In Large Numbers In All…

Q: QUESTION 2 Given the following information, what would be considered to be the positive control in…

A: 2. Positive control in an experiment is a group that receives treatment with a known result and it…

Q: Using the information in the table above, determine if the following statement is TRUE or FALSE.…

A: NOTE: AS PER OUR GUIDELINES WE ARE ONLY ALLOWED TO ATTEMPT FIRST QUESTION, PLEASE RESUBMIT THE OTHER…

Q: Reverse isolation may be used for a person receiving _______________.

A: Reverse isolation is used to protect you from germs when your immune system is not working properly.…

Q: QUESTION: How many bacterial cells are in the salad after that time, assuming the generation time is…

A: One bacterial cell was added to the pasta salad at the beginning, according to the information in…

Q: what is the incident beam  it is the beam of light that exits the sample it is the beam of light is…

A: Spectrophotometry is a technique used to measure light absorption or the amount of chemicals in a…

Q: You perform several tests on an unidentified bacterial species and get the results below. Based on…

A: Bacterial identification is the process of determining the specific type, species, or strain of…

Q: If you were to analyze three separate colonies from the plate above, would you expect them all to be…

A: Microbes, which are tiny and nearly invisible, have had a huge influence on society since the…

Q: These test tubes repre the result of which experiment ? O Sudan test for free c acids O Sudan test…

A: Biomolecules are biological molecules which are essential for certain vital process and growth of…

Q: You perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults:…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From…

A: Serial dilution is a process by which a substance such as a bacterial culture is diluted stepwise in…

Q: You perform the Methyl-Red(MR)& Voges-Proskauer(VP) test and gather the following results:…

A: MR-VP Broth is recommended for use in the performance of the Voges-Proskauer and Methyl Red tests as…

Q: Five dialysis bags, impermeable to sucrose, were filled with various concentrations of sucrose and…

A: Five dialysis bags with varying sugar concentrations are provided. However, the bag's membrane is…

Q: Your first task in the lab is to learn how to culture these cells and begin expanding the culture…

A: Answer:  Yes, the cell culture media need to be changed more often than the other. The open culture…

Q: Multitest systems use a series of miniaturized biochemical tests to determine the identity of an…

A: Biochemical tests are the tests that are specifically carried out on different bacteria for the…

Q: We performed two methods of electrophoresis this semester. List both electrophoresis methods, and…

A: Electrophoresis is the migration of charged particles under the influence of electric current.…

Q: A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the…

A: CFU means colony forming unit. It is a measure of viable bacterial or fungal cells. In case of…

Q: Assume that you are starting with a sample containing 6 billion active cells. Create a dilution plan…

A: Active cells 6 billion Aim is to achieve 30-300 colonies per plate Sample weight 500 mg or 0.5 gm

Q: Before the experiment was run, the apparatus was sterilized and then carefully sealed. Why was this…

A: Sterilization can be classified into physical and chemical methods. Physical methods include the use…

Q: A group of students would like to carry out an experiment to make paper from banana fibre in…

A: Banana fibre, also called as musa fibre, is one of the toughest natural fibres on the planet. The…

Q: Why annealing, denaturation temperatures are different?  Please answer at your own words.

A: Question: Why annealing, denaturation temperatures are different?

Q: Please help me answer how much volume of cell suspension needed (for 5*10^4 cells) in microliters.…

A: For some experiments, it is essential to use the specified number of cells of microbes (or…

Q: Nucleic acids usually migrate towards the negative electrodes. Validate the statement.

A: NUCLEIC ACIDS- They have either single or double-stranded DNA or RNA molecules. The nucleic acids…

Q: A bacteriophage stock with a concentration of 1.45 x 1014 PFU/ml is prepared for an experiment.…

A: The plaque assay is a method of quantifying a viral population and to purify the clonal population…

Q: In the more common protocol for immunofluorescence detection of cellular proteins, an investigator…

A: Antibodies have Fc and Fab parts. The Fab part binds with the epitope of the antigen. The Fc…

Q: In making red blood cell suspension, why do we need to wash red blood cells in saline?

A: Red cell suspension is a common reagent used for many serologic procedures. Red cell suspensions…

Q: When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies…

A: Dilution is the process of decreasing the concentration of the solution. The cells count decreases…

Q: Which two fluorescence-based methods allow localization of proteins in vivo?

A: There are a number of advance microscopy techniques that help in understanding living cells.…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.