Treatment of antibodies with a particular protease clips theheavy chains in half, releasing the two arms of the Y-shapedmolecule. How might the antibodies continue to function?
Q: Which of these decapeptide is the most hydrophobic?
A: 1)Answer is option 1"3". It has total of 7 hydrophobic amino acids which are…
Q: Relatively hydrophobic proteins will require higher amounts of (NH4)2SO4 to precipitate.
A: Amminium sulfate precipitation is a method of protein purification by altering solubility of…
Q: Identify the following by describing their functions: EF-G, EF-Tu, EF-Ts, EF-P, and peptidyl…
A: Elongation factors are a set of proteins that function at the ribosome, during protein synthesis,…
Q: Why is hemoglobin more water soluble than α-keratin?
A: Hemoglobin is a globular protein hence more water soluble. α-keratin is a fibrous protein hence less…
Q: Based on the structure of CBB G-250, explain why it can bind to proteins having basic and…
A: CBB G-250 (Coomassie Brilliant Blue G-250) is a dye used to visualize proteins. There are two types…
Q: Explain how the color change occurs with the biuret reagent in the presence of proteins
A: Protein is a polypeptide chain of amino acid, Biuret test is a kind of biochemical test, used to…
Q: Given Poly-L-Lysine and Epsilon Poly-L-Lysine, which polymer(s) can easily dissolve in a saline…
A: Poly-L-Lysine are several types of lysine as homopolymers.Poly-Lysine enhances electrostatic…
Q: Multisubunit proteins in which some or all of the subunitsare identical are referred to as…
A: Multisubunit proteins are considered as those proteins, which are composed of more than one protein.…
Q: Certain bacterial species have outer layers composed of polymers made of D-amino acids. Immune…
A: The immune system acts as a defense mechanism for the body. It helps to recognize the foreign…
Q: Define the term motilin?
A: The digestive system is made up of the gastrointestinal tract and the accessory organs of digestion…
Q: Identification. Write in CAPITAL LETTERS. Wrong spelling, wrong. Proteins are classified…
A: Protein is a macronutrient that is vital for building mass. It is ordinarily found in animal items,…
Q: 39. The most expensive method to purify the protein is: Affinity Size exclusion RP-HPLC (reversed…
A: Proteins: Proteins are chemical substances that are made up of building blocks that are commonly…
Q: why is hemoglobin more soluble in water than a-keratin?
A: Hemoglobin is a metalloprotein that is responsible for binding oxygen and transporting into cells…
Q: In TLC, why are the colors of the peaks for each amino acid are different depending on the types of…
A: TLC stands for Thin Layer Chromatography. It is a refined technique that is used for isolating or…
Q: Define the term and describe the autoantibodies presentin SLE.
A: Antibodies are Y-shaped compounds produced in the body in response to foreign agents. These are…
Q: 84) An antimicrobial is described as preventing alanine-alanine bridge formation. This means it
A: Antibiotics are antimicrobial substances that are used against bacteria for clearing microbial…
Q: The T-state of hemoglobin is favored over the R-state in blood under which of the listed conditions?
A: Introduction: Hemoglobin is the most abundant porphyrin-containing compound in red blood cells…
Q: Thereareseveralwaystoproducetheaminoacidsthatbuildaprotein.What
A: Amino acids are organic compounds which combine in order to form proteins.After digestion or…
Q: Keratins require the usage of ATP in order to multimerize into an octa-tetramer form.
A: Introduction : Keratin is an important protein that makes up the skin, hair and nails. The protein…
Q: If an antibody binds its antigen with affinity of 150 nanomolar, what is the standard binding energy…
A: The binding and dissociation interactions between an antibody (Ab) and its antigen (An) is shown…
Q: Chymotrypsin treatment of HP peptide yielded free amino acid, dipeptide, tetrapeptide composed of L,…
A: Proteases are the enzymes which hydrolyse proteins into peptides and amino acids. Proteases are…
Q: Apohemoglobin (apoHb) is a dimeric globular protein with two vacant heme‐binding. The preparationof…
A: According to the question, we have to mention that the appropriate method for removing the heme when…
Q: Describe lysine degradation.
A: The amino acid is an organic acid that contains an alpha carboxyl group, alpha amino group, a…
Q: L--L--F--A--I--A--M--F--L--N--I--A--I--A--I What do you think will happen if large concentrations…
A: The given peptide is L--L--F--A--I--A--M--F--L--N--I--A--I--A--I.
Q: The structure of hemoglobin consists of chains. Two of the chains are two are beta proteins. and…
A: Hii... according to our answering policy we will be able to solve only the first three parts. If you…
Q: Explain why bacteria with the gene for the enzyme beta-lactamase are not susceptible to penicillin…
A: A beta-lactam ring is a four-membered lactam a cyclic amide, and beta-lactams are named so because…
Q: Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels. 1.…
A: Non-reducing SDS-PAGE separates based only on the denaturing capabilities of SDS; this allows for…
Q: how/why you will characterize the proteins (whether impurities or dsRed.m1) as it comes off the DEAE…
A: Diethylaminoethyl cellulose (DEAE-C) It is a type of resin that is Hydrophilic, Positively charged,…
Q: What side group reacts with this reducing agent? What does the fact that these peptides both react…
A: There are four levels of protein structure hierarchy: Primary structure, Secondary structure,…
Q: Lead acetate solution is an agents of denaturation. Explain in 10 sentences the effect of each on…
A: Denaturation is the process in which the protein loses its structural configuration and as a result,…
Q: Monoclonal antibodies can be conjugated to an insoluble support by chemical methods. Explain how…
A: The cloning of immune cells results in the generation of similar immunoglobulins called monoclonal…
Q: Treatment of isovaleric aciduria : a. Arginine b. Lysine c. Glycine d. Methionine
A: Isovaleric aciduria occur due to defective catabolism of leucine due to deficiency of enzymes…
Q: Describe the stucture of triacylglycerols (TGA) and explain the hydrolysis of TAGs
A: Carboxylic acid with hydrocarbon side chain are fatty acids. Triacylglycerol are formed from…
Q: Why do we say. Blood Urea, not Serium Urea..... Likewise, why do we say... Serium Kiriatinine and…
A: The nitrogenous end products of metabolism are urea and creatinine. Urea is the major metabolite…
Q: precipitation of proteins is done by all of these except -
A: Proteins are made up of amino acids joined together by peptide bonds. Proteins can be precipitated…
Q: Do all the amino acids with aromatic side chains give positive result to Xanthoproteic acid test.…
A: Amino acids are biologically important as they are the primary building blocks of proteins. The…
Q: Why picric and tannic acid is used for the treatments of burn. Explain this in the concept of…
A: Tannic acid and picric acids are phenolic compounds. These are water-soluble compounds…
Q: он он GalNAC он он Gal но. CH;CONH B(1-) Gal a(13) a(12) B(1-) H.C- a(12) но L-Fuc он A antigen OH…
A: There are four types of blood group which are differentiated according to the antigens present on…
Q: Glucose reacts slowly with hemoglobin and other proteins to form covalent compounds. Why is glucose…
A: Glucose is a monosaccharide with a molecular formula C6H12O6. It is simple sugar easily digestible.…
Q: When a protein denatures in a cell, it tends to assemble into aggregates. Propose an explanation for…
A: Proteins have distinct structures. If they are exposed to specific conditions, they may lose their…
Q: Why are integral proteins more difficult to study then peripheral, and why integral can only be…
A: The ease with which a molecule can flow through a cell membrane is referred to as "permeability".…
Q: Cut the following protein with the Serine Proteolytic enzyme Trypsin. How many peptide fragments are…
A: Proteins are unbranched polymers constructed from 20 standard α-amino acids. They have four levels…
Q: IgG is one of five classes of antibodies that can be produced by our immune system. IgGs have a…
A: Immunoglobulins are heterodimeric proteins with two heavy chains (H) and two light chains (L). They…
Q: Reversible denaturation of the proteins during salting out and precipitation.
A: As different proteins have different compositions of amino acids, different protein molecules…
Q: We note in Figure 10-14 that the structure of glycosphingolipids determines the blood groups A, B,…
A: ABO system of blood group is genetically determined. Genes of the ABO system is present on the…
Q: how do salt bridges that include amino-terminal carbamate stabilize the deoxy form of hemoglobin.…
A: Hemoglobin is a protein which transports oxygen and carbon dioxide to the tissues and to the lungs…
Treatment of antibodies with a particular protease clips the
heavy chains in half, releasing the two arms of the Y-shaped
molecule. How might the antibodies continue to function?
![](/static/compass_v2/shared-icons/check-mark.png)
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
- Digestion of antibodies with mercaptoethanol yields: Select one: 2 Fab, Fc 2 Fab, and 1 Fc 4 polypeptide chainsThe valence of an antibody that is a pentamer is: O 1) 2 O2) 4 O 3) 3 O4) 10A 31-year-old patient develops a severe full-body rash almost immediately after receiving penicillin. Describe what is likely the cause of the rash. [Hint - this is asking about hypersensitivity]
- Observe the structure of antibodies below that has four protein subunits. Explain why we only see two (not four) bands on the SDS PAGE gel.In (b), why would it be more efficient to use labeled anti-human IgG rather than label the patient’s antibodies?Describe the development of antibodies to a specificantigen.
- Consider a pencil-shaped protein with two epitopes, Y (the “eraser” end) and Z (the “point” end). They are recognized by antibodies A1 and A2, respectively. Draw and label a picture showing the antibodies linking proteins into a complex that could trigger endocytosis by a macrophage.Complement activation is a cascade reaction, with each component sequentiallyacting on others, in a similar way to the blood-clotting system. Discuss the effectsthat occur when peptides are generated from the activation by either the classicalor the alternative pathway. This is an immunology questionThe vaccine for Haemophilus influenzae type b is called a conjugate vaccine. It is composed of the tetanus toxoid protein conjugated to the capsular polysaccharide of the H. influenzae type b bacteria. When used to vaccinate infants, the antibody response generated by this vaccine would include 0 0 0 0 antibodies that bind only to the protein-polysaccharide conjugate in the vaccine. antibodies to the tetanus toxoid only. antibodies to the bacterial polysaccharide and the tetanus toxoid. antibodies to the bacterial polysaccharide only.
- What secondary antibody would I need if I purchase a rabbit anti Bax antibody?Why antibodies raised against a native protein sometimes fails to bind to the corresponding denatured protein?Explain your answer.Labs that do a lot of Western blots often have hundreds of primary bodies but only two or three secondary antibodies. Why? I understand that secondary antibody aids in the detection, sorting or purification of target antigens by binding to the primary antibody which directly binds to the target antigen. However, I don't undrstand why there are only two or three secondary antibodies available in the laboratory. Glad if the expert would advise.