How would you measure deamidation in a monoclonal antibody?
How would you measure deamidation in a monoclonal antibody?
A monoclonal antibody (mAb or moAb) is an antibody made up of a particular white blood cell. All subsequent antibodies produced from this route trace back to a single parent cell.
Monoclonal antibodies can have monovalent affinity, binding only to the same epitope (the part of an antigen that is recognized by the antibody). Polyclonal antibodies, on the other hand, bind to multiple epitopes and are normally formed by several different antibody-secreting plasma cell lines.
Monoclonal antibodies are subjected to a wide range of post-translational modifications (PTMs) that cause structural heterogeneity. Deamidation is one such process.
Deamidation refers to the hydrolysis of the amide side chain of Asn and Gln residues to form their corresponding carboxylic acid derivatives.
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