To improve peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following: "2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm³ min¹." Based on the given preparation procedure, identify THREE mistakes that were
To improve peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following: "2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm³ min¹." Based on the given preparation procedure, identify THREE mistakes that were
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:To improve peptide separation by using a HPLC system, trifluoroacetic acid acts
as mobile-phase modifier was added during the preparation of mobile phase.
The preparation was performed by a postgraduate student as following:
"2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a
graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing
the mobile phase was placed in the solvent reservoir and pumping was
commenced at 1.5 cm min-1."
Based on the given preparation procedure, identify THREE mistakes that were
made.

Transcribed Image Text:A chemist attempted to synthesize benzoic acid by the oxidation of benzyl
alcohol. However, a mixture of products was obtained when chromatography
was used to separate them. Only three organic compounds were obtained which
are benzaldehyde, benzyl alcohol and benzoic acid. In addition, three spectra are
shown in Figure 2.
Wavenumber cm
Specrum (a)
4000
3000
2000
1500
1000
500
100-
50-
Spectrum (b)
4000
3000
2000
1500
1000
500
100
50-
Spectrum (c) 4000
3000
2000
1500
1000
500
100-
50-
Figure 2
Match the spectra to their respective compounds with justification.
Transmitance (%)
Transmittance (%)
Transmittance (%)
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