Thanks To improve peptide separation by using a HPLC system, trifluoroacetic acid actsas mobile-phase modifier was added during the preparation of mobile phase.The preparation was performed by a postgraduate student as following:"2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in agraduated flask. To this solution, 50 cm³ of ethanol was added, and after mixingthe mobile phase was placed in the solvent reservoir and pumping wascommenced at 1.5 cm min-1."Based on the given preparation procedure, identify THREE mistakes that weremade. A chemist attempted to synthesize benzoic acid by the oxidation of benzylalcohol. However, a mixture of products was obtained when chromatographywas used to separate them. Only three organic compounds were obtained whichare benzaldehyde, benzyl alcohol and benzoic acid. In addition, three spectra areshown in Figure 2.Wavenumber cmSpecrum (a)40003000200015001000500100-50-Spectrum (b)4000300020001500100050010050-Spectrum (c) 40003000200015001000500100-50-Figure 2Match the spectra to their respective compounds with justification.Transmitance (%)Transmittance (%)Transmittance (%)

The objective of the question is to find the three mistakes that were made while preparing mobile…

To improve peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following: "2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm³ min¹." Based on the given preparation procedure, identify THREE mistakes that were

To improve peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following: "2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm³ min¹." Based on the given preparation procedure, identify THREE mistakes that were

Organic Chemistry

8th Edition

ISBN:9781305580350

Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Chapter14: Mass Spectrometry

Section: Chapter Questions

Problem 14.37P

See similar textbooks

Related questions

Q: The Kc value for Mg3(AsO4)2(s) = 3Mg2+(aq) + 2AsO43-(aq) is 2.1 x 10-20. Determine the Kc for…

A: If the two opposing process in a reversible change are chemical change and the state in this change…

Q: reagent to complete each reaction. Ignore inorganic byproducts. ++ ye ⠀⠀ U 31. Select to Draw 80 HCI…

A: Alkynes undergo addition reactions when treated with Cl2 or HCl or Br2.

Q: 111), (01-1) and ,

A: (111) :

Q: H,0, H,O* (6) 1) ВН, THF (7) 2) H2O2, OH ? (8) (CH3)2C=CH2 (CH3)2CHCH2OH

Q: Compound 3 The M is at 144 m/z. 100- Relative Intensity SIEMELNGA 8 9 8 Compound 4 Relative…

A: The question is asking us to interpret the mass spectrometry data for two compounds, Compound 3 and…

Q: (9.29 × 105/8)-20.81 = with correct sig figs

A: In measurement the number of digits in value is known as significant figure, all non zero values…

Q: Cadii Part C The following ions contain the same number of electrons. Rank them in order of…

A: In the isoelectronic species (same number of electrons), higher the amount of positive charge has…

Q: charting costs ($)- TTT TT 2. On the graph shown to the right: (a) Draw a star (*) on the abscissa.…

Q: You have an unknown with an observed melting point of 123-126"C. Which of the following standards…

Q: Compare the width of PI to the range of the y-value from the sample: min: 3.2% max: 9.0%, range =…

A: To compare the width of PI to the range of the y-value from the sample, we need to know what PI…

Q: Product characterization: 0.91 g of the crude product 0.77 g of the final product M.p. (crude):…

A: Given: Mass of Crude product = 0.77 g Mass of Final product = 0.91 g

Q: Absorbance (arb.units) 2: 04 NO B-Carotene Calibration Curve 2004 6503 UM O 3.26 mM 5,06 nM. y…

Q: What is the role of substance “D" in the reaction for the correct rate law?

A: Concept introduction: A reaction mechanism is a collection of elementary steps that explains how the…

Q: 1. Pereent abundance A. Calculate the average Mass of Ni'? 1 100 60 (242351 20 (10%) 38 62 64…

A: A question based on atomic structure, which is to be accomplished.

Q: In part A.3. as the sample cools, moisture from the atmosphere condenses on the outside of the…

A: In the process described, an evaporating dish containing a water sample is left to cool, during…

Q: edia bo O a. O О С НО НО 1.) 2. НО 3. NaBH, Time left 0:30:20 D НО

A: An arrow always depicts from a region of high electron density to low electron density ; that is…

Q: How would you simplify the following expression?

A: The uncertainty is L213-12π2-L22. We have to simplify the above expression.

Q: Name HI CH3 H-C-C-C- H CH3 Name H-C H HIC 2 H1CIN H C H 3 4 C- | | CH3 H HICIN HIC C₂H5 H 51 H -C-H…

A: According to IUPAC nomenclature firstly select the main chain and then branched chain. and the main…

Q: Li, NH3 ELOH CH3CI AICI3 Br2 hv

A: As per our guidelines we can only solve first three sub-parts of a question. Please resubmit the…

Q: A student weighed 2.0 g crude sample (impure) and found 0.3 g glass and recovered 0.7 g of the…

Q: Draw the starting alkene that would lead to these major products under these conditions.

A: Alkenes are hydrogenated by the reaction with hydrogen in presence of finely divided nickel,…

Q: 2. CoHgO LOD HSP-45-691 0 T 9 4000 T 8 T 3000 1 : T 7 1 6 2000 T 5 ppm : VENUMBER 1 4 1 3 1500 4:2 2…

A: The question is based on the concept of organic spectroscopy. We need to analyse the spectral data…

Q: CI -NO₂ NaOCH₂CH3 heat ?

A: The objective is to determine the product of the given reaction.

Q: 50 Percentage Red No. 40 Soln 100% 80% 60% 40% Table 4.1: Dilutions of Red No. 40 & Absorbance…

A: Detailed answer shown belowExplanation: An experiment to measure the absorbance of Red No. 40 dye at…

Concept explainers

Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

Thanks

To improve peptide separation by using a HPLC system, trifluoroacetic acid acts
as mobile-phase modifier was added during the preparation of mobile phase.
The preparation was performed by a postgraduate student as following:
"2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a
graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing
the mobile phase was placed in the solvent reservoir and pumping was
commenced at 1.5 cm min-1."
Based on the given preparation procedure, identify THREE mistakes that were
made.

A chemist attempted to synthesize benzoic acid by the oxidation of benzyl
alcohol. However, a mixture of products was obtained when chromatography
was used to separate them. Only three organic compounds were obtained which
are benzaldehyde, benzyl alcohol and benzoic acid. In addition, three spectra are
shown in Figure 2.
Wavenumber cm
Specrum (a)
4000
3000
2000
1500
1000
500
100-
50-
Spectrum (b)
4000
3000
2000
1500
1000
500
100
50-
Spectrum (c) 4000
3000
2000
1500
1000
500
100-
50-
Figure 2
Match the spectra to their respective compounds with justification.
Transmitance (%)
Transmittance (%)
Transmittance (%)

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1: Write the objective.

VIEW

Step 2: Find the three mistakes while preparing mobile phase modifier.

VIEW

Solution

VIEW

Step by step

Solved in 3 steps with 5 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

ve Transmittance 0.6 3000 Anthracene INFRARED SPECTRUM P Wavenumber (cm-1) 1000 NIST Chemistry WebBook (https://webbook.nist.gov/chemistry) 1- For the above shown IR-Spectrum (Indicate the structure of the organic molecule). What are the characteristic peaks present? 2- Show (or draw) the IR spectrum, The NMR Spectrum and the Mass spectrum for Methyl ethyl ester. 3- Show or hand draw the IR spectrum, The NMR Spectrum and the Mass spectrum for Acetyl Salicylic acid (commonly known as Aspirin) X
(a) Based on the mass spectrum shown below, 100 73 LOH 80 - A 58 20 - 100 10 20 30 40 50 60 70 80 90 100 110 m/z (i) identify the molecular ion peak of compound A. (ii) explain how the labelled fragments are formed. Relative Intensity
The mass spectrum of compound B is shown below. 100 180 80 181 77 В 182 20 104 25 50 75 100 125 150 175 200 m/z (i) Identify the molecular ion peak of compound B. (ii) Explain the peak at m/z 182 in terms of intensity. (iii) Explain the formation of the other labelled fragments (m/z 180, 104 and 77). Relative Intensity
Select the functional group represented in the following IR spectrum. LOD TRANSMITTANCEI D 4000 O aldehyde carboxylic acid ketone O alcohol ester 3000 2000 HAVENUMBERI-I 1500 1000 500
identify relevant peaks in thediagnostic region as well as the ortho substituted “C-H oop” bending in thefingerprint region. also include both the wavenumber and molecularmotion/functional group in the identification. thanks in advance
1. The two mass spectra below correspond to two isomers of C5H10O2: methyl butanoate and valeric acid. Match the spectrum with the appropriate compound. Place the m/z ratio and the structures (show all atoms) for the labeled fragments in the table below. 100 - Compound MS-NU-1708 Methyl butanoate 80 - 1 60 - 40 - CH3 2 H3C 20 - 10 20 30 40 50 60 70 80 90 100 m/z 100 - Compound MS-NU-3125 3 80 - Valeric acid 60 - 4 40 - H3C 20 - 10 20 30 40 50 60 70 80 90 100 m/z Fragment 1 Fragment 2 Fragment 3 Fragment 4 m/z 74 87 Fragment Relative Intensity
Deduce what is the unkown compounds. Please label the peaks
Which functional group, except alkane, is presence in the following IR spectrum? INFRARED SPECTRUM Relative Transmittance 0.96 0.92 carboxylic acid ether alcohol alkene 3000 2000 Wavenumber (cm-1) m 1000
Based on your interpretation of the mass spectral fragmentation, what are the structures of the two compounds?
The answer is D, could someone please explain it
The mass spectrum of Compound C is shown below: Draw the most likely species responsible for the signals at m/z 91.
The three compounds shown below are structural isomers of each other. Match each compound with its corresponding mass spectrum and draw the fragment ion corresponding to the base peak in each. а. b. ОН H 100 Compound 80 60 Fragment 40 20 - 10 20 30 40 50 60 70 80 m/z 100 Compound 80 - 60 Fragment 40 - 20 10 20 30 40 50 60 70 80 m/z 100 - Compound MS-NW-0661 80 60 Fragment 40 20 10 20 30 40 50 60 70 80 90 100 m/z Relative Intensity Relative Intensity Relative Intensity