Thanks To improve peptide separation by using a HPLC system, trifluoroacetic acid actsas mobile-phase modifier was added during the preparation of mobile phase.The preparation was performed by a postgraduate student as following:"2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in agraduated flask. To this solution, 50 cm³ of ethanol was added, and after mixingthe mobile phase was placed in the solvent reservoir and pumping wascommenced at 1.5 cm min-1."Based on the given preparation procedure, identify THREE mistakes that weremade. A chemist attempted to synthesize benzoic acid by the oxidation of benzylalcohol. However, a mixture of products was obtained when chromatographywas used to separate them. Only three organic compounds were obtained whichare benzaldehyde, benzyl alcohol and benzoic acid. In addition, three spectra areshown in Figure 2.Wavenumber cmSpecrum (a)40003000200015001000500100-50-Spectrum (b)4000300020001500100050010050-Spectrum (c) 40003000200015001000500100-50-Figure 2Match the spectra to their respective compounds with justification.Transmitance (%)Transmittance (%)Transmittance (%)

The objective of the question is to find the three mistakes that were made while preparing mobile…

To improve peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following: "2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm³ min¹." Based on the given preparation procedure, identify THREE mistakes that were

To improve peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following: "2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm³ min¹." Based on the given preparation procedure, identify THREE mistakes that were

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

Thanks

To improve peptide separation by using a HPLC system, trifluoroacetic acid acts
as mobile-phase modifier was added during the preparation of mobile phase.
The preparation was performed by a postgraduate student as following:
"2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a
graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing
the mobile phase was placed in the solvent reservoir and pumping was
commenced at 1.5 cm min-1."
Based on the given preparation procedure, identify THREE mistakes that were
made.

A chemist attempted to synthesize benzoic acid by the oxidation of benzyl
alcohol. However, a mixture of products was obtained when chromatography
was used to separate them. Only three organic compounds were obtained which
are benzaldehyde, benzyl alcohol and benzoic acid. In addition, three spectra are
shown in Figure 2.
Wavenumber cm
Specrum (a)
4000
3000
2000
1500
1000
500
100-
50-
Spectrum (b)
4000
3000
2000
1500
1000
500
100
50-
Spectrum (c) 4000
3000
2000
1500
1000
500
100-
50-
Figure 2
Match the spectra to their respective compounds with justification.
Transmitance (%)
Transmittance (%)
Transmittance (%)

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