Thanks To improve peptide separation by using a HPLC system, trifluoroacetic acid actsas mobile-phase modifier was added during the preparation of mobile phase.The preparation was performed by a postgraduate student as following:"2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in agraduated flask. To this solution, 50 cm³ of ethanol was added, and after mixingthe mobile phase was placed in the solvent reservoir and pumping wascommenced at 1.5 cm min-1."Based on the given preparation procedure, identify THREE mistakes that weremade. A chemist attempted to synthesize benzoic acid by the oxidation of benzylalcohol. However, a mixture of products was obtained when chromatographywas used to separate them. Only three organic compounds were obtained whichare benzaldehyde, benzyl alcohol and benzoic acid. In addition, three spectra areshown in Figure 2.Wavenumber cmSpecrum (a)40003000200015001000500100-50-Spectrum (b)4000300020001500100050010050-Spectrum (c) 40003000200015001000500100-50-Figure 2Match the spectra to their respective compounds with justification.Transmitance (%)Transmittance (%)Transmittance (%)

The objective of the question is to find the three mistakes that were made while preparing mobile…

To improve peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following: "2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm³ min¹." Based on the given preparation procedure, identify THREE mistakes that were

To improve peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following: "2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm³ in a graduated flask. To this solution, 50 cm³ of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm³ min¹." Based on the given preparation procedure, identify THREE mistakes that were

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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3
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All changes save 7. When two solutions are mixed, a color change occurs. The data tables show the time between mixing and the color change for two sets of conditions. For Condition One, the solution concentrations were constant and temperature varied. For Condition Two, the temperature was constant and concentrations varied. Condition One: Concentration Time for Temperature (°C) Sample Color to Change 1 10° 36 sec 22° 14 sec Condition Two: Temperature Time for Concentration Sample Color to % Change 1. 100% 15 sec 2. 50% 24 sec Which of these statements is true according to the data? O The reaction rate is greater at 22°C than at 10°C. O Reducing the temperature increases the rate of the reaction. The reaction is affected by changes in temperature not by changes in concentration. O Decreasing the concentration increases the reaction rate. PREVIOUS 17 of 25 NEXT SAVE & EXIT
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Question 10 What is the correct equation for percent difference? |measured+accepted| |measured-accepted|/2 х 100 |value 1-value 2|| |value 1+ value 2|/2 × 100 |mеasured-accерeted] |accepted| х 100 |value 1 -valuие 2| х 100 laccеpted
The inital points are ( 0, 27.12) and (0.00250 , 5.17)
Data Analysis: 1. Create an Absorbance (Y) vs. Concentration (X) graph using data from the six standard solutions above. 2. Perform a linear trendline analysis of your six data points. Display the equation and R² value on the graph with a minimum of 8 decimal places. 3. On the same graph, perform a 2nd order polynomial trendline analysis of your six data points. Display the equation and R² value on the graph with a minimum of 8 decimal places. 4. Turn in your graph as the last page of this report. 5. On a separate piece of paper calculate the concentration of the unknown solution using both the linear and 2nd order polynomial trendline equations (Show all work) 6. Choose the best unknown concentration from #4 above and report it with three significant figures in the unknown data table above.
ASSAY Calibration solutions Calibration solutions of naproxen in the range 5 – 25 μg/mL were prepared. Sample preparation 20 tablets weighing 12.3819 g were crushed to a fine powder. A portion of the powder (145.4 mg) was shaken with approximately 150 mL of acetic acid (0.05 M) for 5 min and then made up to volume in a 250 mL volumetric flask (stock solution). Approximately 50 mL of the stock solution was filtered and a 25 mL aliquot was diluted to 100 mL in a volumetric flask. 10 mL of the resulting solution was further diluted to 100 mL with acetic acid (0.05 M). Analysis The standards and sample solutions were analyzed by HPLC under the following conditions: Column: octadecylsilyl (ODS), 4.6 mmx150 mm, mobile phase: acetonitrile : 0.05 M acetic acid (85:15), flow rate: 1 ml/min, UV detection at 243 nm. Results: A calibration curve of concentration versus peak area was constructed for the standard solutions and gave the straight-line equation: y = 3555.6x + 85, r = 0.9999 The area…