Absorbance (arb.units) 2: 04 NO B-Carotene Calibration Curve 2004 6503 UM O 3.26 mM 5,06 nM. y (105612 M)x-0.1736 9294M 404 104 FRE 181 1211 Concentration (M) 1004 à Abserbence Carbunits) 0 0.25 200 A student creates a standard curve of B-Carotene and plots the line of best fit. They then measure the absorbance of a solution of B-Carotene with an unknown concentration. What is the concentration of the sample? You can assume they used a cuvette with a pathlength of 1 cm. 500 Wavelength (nm)

Absorbance (arb.units) 2: 04 NO B-Carotene Calibration Curve 2004 6503 UM O 3.26 mM 5,06 nM. y (105612 M)x-0.1736 9294M 404 104 FRE 181 1211 Concentration (M) 1004 à Abserbence Carbunits) 0 0.25 200 A student creates a standard curve of B-Carotene and plots the line of best fit. They then measure the absorbance of a solution of B-Carotene with an unknown concentration. What is the concentration of the sample? You can assume they used a cuvette with a pathlength of 1 cm. 500 Wavelength (nm)

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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The concentration of nitrite (NO2) in the unknown sample was determined by a direct (ordinary) calibration plot as shown below: 0.300 Absorbance at 520nm 0.250 0.200 0.150 0.100 0.050 0 0.564 µg/ml 0.200 μg/ml 0.754 µg/ml 0.902 µg/ml y = 0.1793x + 0.0763 R² = 0.9969 0.1 0.2 0.801 μg/ml If the absorbance of the unknown sample was measured 0.238 at 520 nm, what would be the concentration of nitrite? 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 Nirite (µg/ml)
Sally obtains a standard calibration curve for their assigned food dye by plotting absorbance versus concentration (in M) of known solutions and finds the following slope and intercept: y = 1.646x + 0.026 Sally finds that her original Kool-aid sample is too concentrated, so they dilute it by transferring 13.00 mL of the original Kool-aid sample into a new container and diluting to a total volume of 37.00 mL. If the absorbance of the dilute Kool-aid sample at the appropriate wavelength for their assigned dye is 0.950, determine the concentration (in M) of the assigned food dye in the original Kool-aid sample. Report answer with three places after the decimal.
3. An indicator is a dye whose spectrum changes with pH. Consider the following data for the absorption spectrum of an indicator (pK = 4) in its ionized and nonionized form. InH+ → In + H* Molar absorptivity, ɛ (M1.cm1) InH+ (nm) In 400 10,000 420 15,000 2,000 440 8,000 8,000 460 12,000 480 3,000 The absorbance of the indicator solution is increased in a 1-cm cell and found to be 400 420 440 460 480 (nm) A 0.250 0.425 0.400 0.300 0.075 a. Calculate the pH of the solution. [4.0] b. Calculate the absorbance of the solution at 440 nm and pH 6.37 for the same total indicator concentration. [0.400] c. If you want to measure the quantum yield of fluorescence of In and H* independently, what wavelength would you choose for exciting In H+? For exciting In? Why?
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TScholarsh X = Report - X Grammar X ent/takeCovalentActivity.do?locator=assignment-take 2req = 2req =2req 5 SM M S s2req O ts 2req ts M (M) ots ots O NGLSync X OWLv2 | X Cengage X * 0 Use the References to access important values if needed for this question. What is the calculated value of the cell potential at 298 K for an electrochemical cell with the following reaction, when the H₂ pressure is 7.74x10-3 atm, the H+ concentration is 1.42M, and the Ni2+ concentration is 4.99 × 10-¹M? 2H+ (aq) + Ni(s) → H₂(g) + Ni²+ (aq) Ecell = V The cell reaction as written above is spontaneous for the concentrations given: Submit Answer Retry Entire Group 9 more group attempts remaining a #3 O C 73°F E 56 0 Update 1:43 PM 5/27/2022 Ę X
During an assay of paracetamol by UV an analyst prepares a series of calibration solutions containing approximately 3-15 mcg/mL paracetamol as follows: 140.1 mg of pure paracetamol was added to a 200 mL volumetric flask. 0.1 M NaOH (50 mL) was added, the mixture well shaken and made up to volume with deionized water 10 mL of the above solution was diluted to 100 mL 1)What is the concentration of the final diluted solution in mcg/mL?(1 decimal) 2) What volume of the diluted solution is required to prepare 100 mL of a 8 mcg/mL standard solution? (1 decimal)
Data Set Exp.5 Use the following data to determine the mass percent of the active ingredient in Aspirin a) Preparation of solutions: volume of salicylic acid volume of FECL3 solution SA1 1ml 9ml SA2 3.5ml 6.5ml SA3 6.5ml 3.5ml SA4 1ml 9ml The concentration of salicylic acid= 0.000725M b) Preparation of "STOCK ASA UNKNOWN" The mass of aspirin= 0.135 g The volume of stock solution of aspirin= 100ml The dilution factor for the stock solution= 50/2 Molar mass of SAS = 180.157 g/mole c) Measurements of Absorbance: Solution Absorbance SA1 0.154 SA2 0.524 SA3 0.789 SA4 0.989 The unknown sample 0.259
= 1 5 Absorbance vs. Concentration y = 0.2755x 0.2- 0.1- 1.400 2.000 1.800 1.600 1.000 0.200 1.200 0.400 0.600 Concentration (M) Using the calibration curve, calculate the molar concentration for a solution with a measured absorbance of 0.374. Round your answer to 3 significant digits Concentration = M X Sub Absorbance 0.7- 0.6- 0.5 0.4- 0.800
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Amax for Red 40 Solution number 1 2 3 4 Concentration 5x105m 2.5x105m Concentration of Red 40 in Gatorade Based on calibration curve, extinction coefficient for Red 40 Absorbance of Red 40 in Gatorade at max Absorbance at max 1.2939.0 .637 5020am 1.25x105m.347 500.0mm 6.25x10m 190 soonm So0nm 2.443
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Cengage Learnir × | Cengage Learnir x | Cengage Learnir X OWLv2 | Online X × Ar Ex3 CH 14 and 15 prod03-cnow-owl.cengagenow.com/ilrn/takeAssignment/takeCovalentActivity.do?lo Question 1 1pt This question has multiple parts. Work all the parts to get the most points. Question 2 1pt Question 3 Question 4 2 pts 2 pts Data in the table were collected at 540 K for the following reaction: Question 5 1 pt CO(g) + NO2(g) → CO2 (g) + NO(g) Initial concentration (mol/L) Initial rate Question 6 Question 7 2 pts 1pt [CO] [NO2] (mol/L. h) 5.1 × 10-4 Question 8 Question 9 2 pts 1pt Question 102 pts Question 111pt 0.35 x 10 4 3.9 × 10-8 5.1 x 10-4 0.70 × 10-4 7.9 x 10-8 5.1 × 10-4 0.18 × 10-4 2.0 × 10-8 1.0 × 10-³ 0.35 × 10-4 7.7 × 10-8 Question 12 1 pt 1.5 x 10-3 0.35 × 10-4 1.2 × 10-7 Question 13 1 pt Question 14 1pt Question 151 pt a Determine the reaction order with respect to each reactant. Order with respect to CO: 1 ✓ ✓ 4 Order with respect to NO2: 1 V✓ Progress: 13/15 items Due Mar 28 at…