How to find any evidence of cantamination or degradation of DNA in the DNA profiles you examined?
how important is the detabase that is used to determine allele frequencies in DNA profiling cases
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DNA STRUCTURE-
DNA structure is given by Watson and Crick, who proposed the double-helical structure of DNA in which DNA is twisted in a helical form having four nitrogenous bases- Adenine, Guanine, Cytosine, and Thymine.
These bases are joined together by a Phosphodiester bond having a sugar-phosphate bone.
DNA is a carrier of hereditary information from one generation to another for continuity of life.
For identifying the contamination in DNA. DNA Fingerprinting or DNA Profiling technique is used.
TECHNIQUE-
For this only a small amount of DNA like blood, semen or hair part is needed. Steps involved are:-
1) DNA that was extracted is centrifuged at a high speed rate.
2) Fpr DNA amplification, Polymerase chain reaction process is used for making multiple copies of the isolated DNA.
3) For restriction fragment length analysis, restriction enzymes are used to cut DNA into fragments.
4) Now these pieces of DNA are introduced into gel through electrophoresis on agarose polymer gel and they can be seen by using dye that glows under ultraviolet radiation.
5) By using alkaline chemicals double stranded DNA splits in Single stranded DNA.
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