To calculate the isoelectric point of amino acids having other ionizable functional groups, we must also take into account thepk, of the additional functional group in the side chain.For an acidic amino acid (one with an additional acidicOH group):For a basic amino acid (one with an aditional basic NHgroup):pK, (a-COOH) + pK, (second COOH)- pKa (a-NH3") + pK, (side chain NH)pl =a. Indicate which pK, values must be used to calculate the pl of each of the following amino acids: [1] glutamic acid;[2] lysine; [3] arginine.b. In general, how does the pl of an acidic amino acid compare to that of a neutral amino acid?c. In general, how does the pl of a basic amino acid compare to the pl of a neutral amino acid?

The isoelectric point is amino acid is the pH at which the amino acid carries no net electrical…

Answered: To calculate the isoelectric point of…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Using the picture provided, match the correct name of each of the functional groups highlighted in blue.
Glutamine is an amino acid that has -CH₂-CH2-CO-NH₂ as its R group. The R group of the amino acid isoleucine is -CH2-CH-(CH3)2. If these amino acids were in a globular protein in aqueous solution, where would they most likely be? O Both glutamine and isoleucine would be in the interior and on the exterior of the globular protein. O Glutamine would be in the interior, and isoleucine would be on the exterior of the globular protein. O There isn't enough information O Both glutamine and isoleucine would be on the exterior of the globular protein. O Isoleucine would be in the interior, and glutamine would be on the exterior of the globular protein.
After staining an SDS-PAGE gel with Coomassie Blue G-250, the protein bands are visualized by de-staining the gel in a Coomassie Blue G-250 de-staining solution. This solution is made up of 10% acetic acid, 50% methanol, and 40% distilled water. How much of each of these components do you need to prepare 5 liters of Coomassie Blue G-250 de-staining solution?
Provide the correct three-letter abbreviation for the following amino acid: O || H₂N-CH-C-OH I CH₂ CH₂ C=O I NH₂
1) Indicate the total charge of the peptide at pH 7.0, 9.0, and 11.0 2) Determine the isoelectric point (pI) for this peptide. Show the relevant pKa’s and your calculation. 3) What is the fraction of the peptide that has the N-terminal amino group deprotonated at pH 9.
If you were given the abbreviations for 4-6 amino acids, could you draw the polypeptide chain AND determine its isoelectric point? Do this for the amino acid Asp-Arg-Val-Tyr-Ile
A HEPTAPEPTIDE that punctures the bacterial cell wall has just been recently isolated from the venom gland of a frog. Amino acid analysis of the peptide reveals the following information below: (NOTE: when the sequence is not known, a comma separates the amino acids) Hydrezine Acid Hydrolysis (6 N HC) at 10 C Heptapeptide (2) Arg. Pro, Phe, Tyr, Ala, Lys 2,4 dinitrafluerobenzene DNP-Phe Lys and modified free amino acids Peptide 1 (Ala, Lys) Cleovage with Trypsin, then chrometogrophy Peptide 2 (Pro, Arg, Tyr) Peptide 3 (Phe, Arg) Phe Peptide 4 (Arg. Tyr) Clevege with Chymetrypsin, then cromategrphy Peptide 5 (Lys, Pro, Ala, Arg)
if the tetrapeptide shown in the binding site above (Gly-Ser-His-lle) were in an aqueous solution at pH=6.2, what fraction of peptide molecules would have a +1 charge?
Consider the following tetrapeptide, written in a sequence according to normal convention, from left to right: Residue 1 is acidic; Residue 2 is proline (a large hydrophobic amino acid); Residue 3 is cysteine (an amino acid containing a -CH2SH R-group); Residue 4 is basic. Which of the following four general statements is TRUE regarding this tetrapeptide? This tetrapeptide probably is not part of an alpha-helix, since the large size of the proline would disrupt the consistency of the spiral shape. Residue 3 belongs to the amino acid category known as "basic." This tetrapeptide has the ability to form a disulfide linkage with an identical tetrapeptide. The acidic residue (Residue #1) is at the amino terminus of this tetrapeptide. 3 of these 4 responses are correct
Draw the Fischer projections representing the L forms of the following amino acids at pH higher than their isoelectric point. a) cysteine b) alanine

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