The solvent in this chromatography experiment is 75% butanol, 12% acetic acid, and 13% water. Is the solvent mostly polar or nonpolar? Explain your answer. answer and the table above, which amino acid(s) will travel furthest in the solvent? Based on your

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A: Biomolecules that contain both an amino group and a carboxyl group are known as amino acids.…

Q: 2. You made a pH=9.8 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in…

A: In order to solve (d), first we need to calculate (c). So, here is a brief and comprehensive…

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A: A percentage solution can be defined as the amount of volume of a particular chemical compound that…

Q: Given a stock protein solution with a concentration of 3 mg/ml, determine the protein concentration…

A: Stock solutions are used in many laboratory procedures, in order to save space for keeping larger…

Q: protein must be conformationally stable protein may be denatured analysis requires information about…

A: Protein structure, function, and characteristics are only fully understood through the use of…

Q: please draw anr label the 20 amino acids found in proteins as they would exist in an aqueous…

A: Amino acids are biomolecules that have an amino group and a carboxyl group linked to the same carbon…

Q: Describe and explain sources of errors in this experiment. Provide practical suggestions for…

A: Protein solubility refers to the ability of a protein to dissolve in a solvent such as water, and is…

Q: Why does adding acetic acid to the milk improve the extraction efficiency of the pentobarbital? You…

A: In liquid–liquid (solvent) extraction, the solute partitions itself between two immiscible phases.…

Q: Indicate which base is (or bases are) the complementarypaired base(s) for each of the…

A: Answer: Introduction: DNA - Deoxyribonucleic acid is a molecule that is used for storage of genetic…

Q: You ran a gel using SDS-PAGE. Total molecular mass of your unknown protein (determined via gel…

A: To determine the molecular weight of subunit C from the SDS-PAGE data, we'll use the provided…

Q: Electrophoresis is the movement of molecules within a matrix (paper, gel) to which an electric field…

A: Amino acids are molecules composed of an alpha carbon bonded to to 4 valencies. They are;…

Q: Use excel to plot the following titration data. Once you have done your plot, make sure to label the…

A: pH = If [H+] concentration in a solution is high, the pH will be low and the solution will be…

Q: You have a separate aqueous solution of the peptide DREAMY that is at a pH of 1.0. You then proceed…

A: pH = 1/log [H+]If [H+] concentration in a solution is high, pH will be low and the solution is…

Q: You are working in the lab with a polypeptide that is present in solution at 1 mg/mL. The…

A: Molarity (M) is the amount of a substance in a certain volume of solution. Molarity is defined as…

Q: A 0.5µM solution of the protein in 75mL is what you want. In micrograms, how much of the protein do…

A: Molar concentration is a measure of the concentration of a solute in solution with respect to the…

Q: In isoelectric focusing, a protein sample is applied at the end of a gel with an immobilized pH…

A: In isoelectric focusing, a protein sample is applied at the end of a gel with an immobilized pH…

Q: Write down the values in bold print. You are isolating a protein with a pl of 8.5 using cation…

A: As given in the question, a protein with pI of 8.5 was isolated using cation exchange…

Q: A protein has a molecular weight of 24kDa.  You want a 0.5µM solution of the protein in 75mL.  How…

A: Given information Protein molecular weight- 24kDa The concentration of the solution to be made-…

Q: You have a stock solution of protein that you would like to dilute. How much stock protein do you…

A: Dilution is a process to lower the concentration the concentration of a solute by the addition of…

Q: A Transmission Electron Microscopy image of Gold nanoparticles is shown below. Name a physical or…

A: Introduction Nanoparticles are one-of-a-kind structures with one-of-a-kind features due to their…

Q: Use any three amino acids from the picture below to form a tripeptide on and then upload the file…

A: Peptide or proteins is the sequence of amino acids which are linked by the formation of peptide bond…

Q: chromatography

A: Hydrophobic interaction chromatography segregates molecules by their degree of hydrophobicity.The…

Q: Describe the mechanism behind the hydrophobic effect. Support your description with one drawing.

A: The hydrophobic effect is the tendency of nonpolar molecules to aggregate in aqueous solutions. This…

Q: Do question 1)

A: Ethanolamine (a):Functional Groups:Hydroxyl group (-OH)Amino group (-NH2)Chiral Carbon: None (All…

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Q: Consider a mixture comprised of the proteins attached: Protein that will most strongly bind to an…

A: Anion exchange chromatography is a type of ion exchange chromatography, used to separate molecules…

Q: 1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL…

A: The four types of biological macromolecules are nucleic acids proteins lipids and carbohydrates.…