A student accidentally added sodium hydroxide (NaOH) to their LB/AMP/ARA total protein sample. Would the sample have fluorescence (glow in the dark)? Why or why not? Briefly justify your answer
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A student accidentally added sodium hydroxide (NaOH) to their LB/AMP/ARA total protein sample. Would the sample have fluorescence (glow in the dark)? Why or why not? Briefly justify your answer.
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- A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, and phosphatidylcholine was applied to a hydrophobic interaction chromatography column. The column was washed with a high salt buffer and the lipids were eluted with decreasing salt concentrations. In what order would the lipids be eluted from the column? Explain your answer.a MD order 15 millimoles of potassium phosphate (MW 136). How any milligrams are need for this does. **Do not round your answer**Choices are: Fraction A Fraction B Fraction C Fraction D None of the above All of the above I selected A and was incorrect, I am now lost on how to go about this. Thank You!
- Given a stock protein solution with a concentration of 3 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.A researcher is preparing a reaction mixture to test the activity of a protein. They combine at room temperature the required reaction components, which contained in a final 150 ml volume 200 mM NaCl and unknown concentrations of weak acid and conjugate base of a buffer compound. They are using a pH meter to determine the acidity of the solution and observe the following pH value that is displayed by the device. Can you determine the hydrogen ion [H+] concentration of the solution? Provide the value with three decimal places and an appropriate unit in the answer box. Note: You may need to round the numbers to get the required answer. N 3.35A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, phosphatidylserine, andphosphatidylethanolamine was applied to a hydrophobic interaction chromatography column. Thecolumn was washed with a high salt buffer, and the lipids were then eluted with decreasing saltconcentrations. In what order would the lipids be eluted from the column? Explain your answer.
- if you had a protein sample solution of unknown concentration which gave an absorbance of 0.992 after the two-fold dilution (25 μL water + 25 μL sample). If the standard curve we constructed. what would you need to do to your sample in order to find its protein concentration more accurately?A researcher prepares 100 mL of aqueous solution containing 0.1 g of a protein. The researcher then uses an experimental apparatus ,where he notes that, at room temperature (300K) and atmospheric pressure (101.325 kPa), distilled water flows naturally into the solution compartment. However, when he applies an extra pressure of 1000 Pa he observes that the flow of distilled water ceases and the system is in balance. Calculate the molar mass of this protein, in kDa (1 Da = 1 g/ mol); Why does the flow of distilled water cease with the application of the pressure of 1000 Pa? Explain in terms of potential chemicals. %3DConsider the given data (Solubility Tests, Solubility Group, Chemical Tests, and Functional Group) in the experiment. Based on the results of the solubility tests and chemical tests, what is the most probable structure of C4H6O2? Draw your answer. You may draw the compound using line-bond formula OR Lewis structure.
- Sketch the appearance after visualization of a protein mixture containing the seven proteins above when subjected to two-dimensional (2D) gel electrophoresis.A researcher is preparing a reaction mixture to test the activity of a protein. They combine the required reaction components, which contained in a final 100 ml reaction volume 200 mM NaCl, unknown concentrations of acetic acid and acetate anions and a total [H+] concentration of 790 ricromolar. Can you determine the pH of the solution? Provide the answer to one decimal place. Note: You may need to round the numbers to get the required answer. 100 Strips pH indicator strips non-bleeding pH pH 0-14 EM-Reagents Dip in-read while still moist. immerse in weakly-buffered solutions une there is no further color change (1-10 min) 3 7 5 6True or False: Beta sheets are helical structures please explain the answer, thanks