Detergents disrupt hydrophobic interactions by coating hydrophobic molecules with molecules that have a hydrophilic surface. When hemoglobin is treated with a detergent, the four polypeptide subunits separate and become random coils*. Explain this observation.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Detergents disrupt hydrophobic interactions by coating hydrophobic molecules with molecules that have a hydrophilic surface. When hemoglobin is treated with a detergent, the four polypeptide subunits separate and become random coils*. Explain this observation.
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Other proteins, such as antibodies, have polypeptide subunits that behave differently. They lose function when treated with detergents, but they do not separate and become random coils. What could cause this difference: how could they remain together but not have activity?
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