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The liver is a major storage site for glycogen. Purified from two samples of human liver, glycogen was either treated or not treated with a -amylase and subsequently analyzed by SDS-PAGE and western blotting with the use of antibodies to glycogenin. The results are presented in the adjoining illustration on the next page. (a) Why are no proteins visible in the lanes without amylase treatment? (b) What is the effect of treating the samples with a - amylase? Explain the results. (c) List other proteins that you might expect to be associated with glycogen. Why are other proteins not visible?

The liver is a major storage site for glycogen. Purified from two samples of human liver, glycogen was either treated or not treated with a -amylase and subsequently analyzed by SDS-PAGE and western blotting with the use of antibodies to glycogenin. The results are presented in the adjoining illustration on the next page. (a) Why are no proteins visible in the lanes without amylase treatment? (b) What is the effect of treating the samples with a - amylase? Explain the results. (c) List other proteins that you might expect to be associated with glycogen. Why are other proteins not visible?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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38. Glycogen isolation 1. The liver is a major storage site for glycogen. Purified from two samples of human liver, glycogen was either treated or not treated with a -amylase and subsequently analyzed by SDS-PAGE and western blotting with the use of antibodies to glycogenin. The results are presented in the adjoining illustration on the next page.

(a) Why are no proteins visible in the lanes without amylase treatment?

(b) What is the effect of treating the samples with a - amylase? Explain the results.

(c) List other proteins that you might expect to be associated with glycogen. Why are other proteins not visible?

 

 

642 CHAPTER 21 Glycogen Metabolism Kilodaltons 212 158 116 97- 66- 56- 43- 37- Sample 1 Sample 2 a-Amylase [Courtesy of Dr. Peter J. Roach, Indiana University School of Medicine.]
Transcribed Image Text:642 CHAPTER 21 Glycogen Metabolism Kilodaltons 212 158 116 97- 66- 56- 43- 37- Sample 1 Sample 2 a-Amylase [Courtesy of Dr. Peter J. Roach, Indiana University School of Medicine.]
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