The graph below shows the results of a FRAP analyses studying Membrane Protein 1-4 (MP1-MP4). Work with your group to answer the questions to the right of each graph. MP1 1. Label the graph with an arrow to show when the laser bleached the 1.0- fluorescent protein (MP1). 0.8- 0.6- 2. Did the MP1 protein move to restore its previous distribution? YES // NO (circle one) 0.4- 0.2- 3. For question 2, what was your reasoning for selecting your answer? 50 100 150 200 250 time (seconds) MP2 4. Did MP2 move to restore its previous distribution? 1.0- YES // NO (circle one) 0.8- 0.6- 5. How does MP2's movement compare to that of MP1? 0.4- faster // same // slower (circle one) Why do you think this is true? 0.2- 50 100 150 200 250 time (seconds) MP3 1.0- 6. Did MP3 move to restore its previous distribution? YES // NO (circle one) 0.8- 0.6- 7. For question 6, what was your reasoning for selecting your answer? 0.4- 0.2- 50 100 150 200 250 time (seconds) MP4 1.0- 8. Did MP4 move to restore its previous distribution? YES // NO (circle one) 0.8- 0.6- 9. Use your knowledge of membrane proteins to propose a model that explains why proteins would show the FRAP pattern of MP4 0.4- 0.2- 50 100 150 200 250 time (seconds) Normalized Fluorescence Intensity Normalized Fluorescence Intensity Normalized Fluorescence Intensity Normalized Fluorescence Intensity

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The graph below shows the results of a FRAP analyses studying Membrane Protein 1-4 (MP1-MP4). Work with
your group to answer the questions to the right of each graph.
MP1
1. Label the graph with an arrow to show when the laser bleached the
1.0-
fluorescent protein (MP1).
0.8-
0.6-
2. Did the MP1 protein move to restore its previous distribution?
YES // NO (circle one)
0.4-
0.2-
3. For question 2, what was your reasoning for selecting your answer?
50
100
150
200
250
time (seconds)
MP2
4. Did MP2 move to restore its previous distribution?
YES // NO (circle one)
1.0-
0.8-
0.6-
5. How does MP2's movement compare to that of MP1?
0.4-
faster // same // slower (circle one)
Why do you think this is true?
0.2-
50
100
150
200
250
time (seconds)
MP3
1.0-
6. Did MP3 move to restore its previous distribution?
YES // NO (circle one)
0.8-
0.6-
7. For question 6, what was your reasoning for selecting your answer?
0.4-
0.2-
50
100
150
200
250
time (seconds)
MP4
1.0-
8. Did MP4 move to restore its previous distribution?
YES // NO (circle one)
0.8-
0.6-
9. Use your knowledge of membrane proteins to propose a model that
explains why proteins would show the FRAP pattern of MP4
0.4-
0.2-
50
100
150
200
250
time (seconds)
Normalized Fluorescence Intensity
Normalized Fluorescence Intensity
Normalized Fluorescence Intensity
Normalized Fluorescence Intensity
Transcribed Image Text:The graph below shows the results of a FRAP analyses studying Membrane Protein 1-4 (MP1-MP4). Work with your group to answer the questions to the right of each graph. MP1 1. Label the graph with an arrow to show when the laser bleached the 1.0- fluorescent protein (MP1). 0.8- 0.6- 2. Did the MP1 protein move to restore its previous distribution? YES // NO (circle one) 0.4- 0.2- 3. For question 2, what was your reasoning for selecting your answer? 50 100 150 200 250 time (seconds) MP2 4. Did MP2 move to restore its previous distribution? YES // NO (circle one) 1.0- 0.8- 0.6- 5. How does MP2's movement compare to that of MP1? 0.4- faster // same // slower (circle one) Why do you think this is true? 0.2- 50 100 150 200 250 time (seconds) MP3 1.0- 6. Did MP3 move to restore its previous distribution? YES // NO (circle one) 0.8- 0.6- 7. For question 6, what was your reasoning for selecting your answer? 0.4- 0.2- 50 100 150 200 250 time (seconds) MP4 1.0- 8. Did MP4 move to restore its previous distribution? YES // NO (circle one) 0.8- 0.6- 9. Use your knowledge of membrane proteins to propose a model that explains why proteins would show the FRAP pattern of MP4 0.4- 0.2- 50 100 150 200 250 time (seconds) Normalized Fluorescence Intensity Normalized Fluorescence Intensity Normalized Fluorescence Intensity Normalized Fluorescence Intensity
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