The following question tests your lab skills as well as basic knowledge understanding: In Lab1- KHP Determination,  0.85 g moisturized NaOH (M.W.= 40.01 g/mol) solid was dissolved in 500.0 mL of water.  24.21 mL of this NaOH solution was consumed to reach the equivalence point when titrated with 0.2021 g of pure KHP (M.W.=204.22 g/mol. Solid was fully dissolved in 50 mL deionized water).

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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2. The following question tests your lab skills as well as basic knowledge understanding: In Lab1- KHP Determination,  0.85 g moisturized NaOH (M.W.= 40.01 g/mol) solid was dissolved in 500.0 mL of water.  24.21 mL of this NaOH solution was consumed to reach the equivalence point when titrated with 0.2021 g of pure KHP (M.W.=204.22 g/mol. Solid was fully dissolved in 50 mL deionized water).

(a). Please write the reaction equation (balanced).  Then calculate the precise concentration of NaOH solution. List all steps.

 

 

 

 

 

 

(b). What are the primary standard and secondary standard material in the above operation respectively?

 

 

 

 

 

(c). During the above operation, what glassware/tools were used to measure 0.85 g NaOH, to store 1 liter NaOH solution, to measure pure KHP solid, to conduct the titration, respective ?

 

 

 

 

 

 

 

(d). Can this titration be used to measure the pH value of an unknown solution?  Why or why not?

 

 

 

 

 

(e). Did we use a pH buffer solution in the above titration?   Why or why not?

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