The following data are from the HPLC assay of paracetamol 500 mg tablets using the calibration curve method: Weight of active ingredient in powdered tablets taken for analysis: 180 mg Initial extraction volume: 200 mL • Dilution steps: 10 mL into 100 mL, then 5 mL into 100 ml . Linear equation: y = 12000x - 50 %3D Area of chromatographic peak for sample is 4750. What is the theoretical concentration in final solution in mg/100 mL? (2 decimal places). Please do not write the units, just numerical value. Answer:

The following data are from the HPLC assay of paracetamol 500 mg tablets using the calibration curve method: Weight of active ingredient in powdered tablets taken for analysis: 180 mg Initial extraction volume: 200 mL • Dilution steps: 10 mL into 100 mL, then 5 mL into 100 ml . Linear equation: y = 12000x - 50 %3D Area of chromatographic peak for sample is 4750. What is the theoretical concentration in final solution in mg/100 mL? (2 decimal places). Please do not write the units, just numerical value. Answer:

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

Retention volume in a chromatographic experiment 1) increases with decreasing length of the column and lower flow rate of eluent 2) decreases with decreasing length of the column at constant flow rate of eluent 3) decreases with increasing column length at constant flow rate of eluent 4) increases with increasing length and higher flow rate eluent 个个 chromatographic
A mixture of species of A, B, and C is injected into a liquid chromatographic column with length of 20.0 cm. The obtained separation results are listed as follows: Please calculate the selectivity factor for species A and B? Please keep your answer to two decimal places. I would need help with these question and the answer is 1.39 (I don't know how to get it)
1 24) The method of internal standard was used to analyze and measure the concentration of chemical X by GC A known mixture of compounds X and Y (internal standard) gave the following chromatographic results: Compound X Y Concentration (M) 6.3 x 10-8 2.0 x 10-7 a) 2.0 x 10-6 M b) 8.4 x 10-7 M c) 6.6 x 10-7 M d) 4.1 x 10-7 M e) 2.9 x 10-7 M Peak Area 395 787 Then, an unknown was analyzed as follows. 3.0 mL of unknown X was treated with 0.100 mL of 1.6 x 10-5 M Y and the mixture diluted to 10.0 mL final volume. Peak areas were 633 (for X) and 520 (for Y). What is the concentration of X in the unknown. (The original 3.00 sample?)
With the following information for a GC analysis using an internal standard, calculate the percentage of alcohol in the sample. The 1% ethanol standard sample has an ethanol peak area of 711 and a 1-propanol peak area of 9253; 3% is 3536 and 14432 for ethanol and 1-propanol respectively; 5% is 3506 and 10693; 7% is 5874 and 10847 and 9% is 5203 and 7522. The beer sample has an ethanol peak area of 5471 and 1-propanol of 14384. 4.95% 5.15% 5.05% 5.25%
4. The paper chromatogram of an unknown mixture is shown below: final solvent level 5.3 ст 6.0 cm B 2.0 cm A 1.0 cm origin A. Using the measured distances find the retention factor of components A, B, and C. B. If the paper was run using hexane as the solvent, (a) which compound is the most polar? (b) the least polar? Explain your answer. C. What would you expect to happen to the Rf values if acetone was used instead of hexane as the solvent?
A new method is proposed to simultaneously detectand separatefour foodadditives by reversed-phase liquid chromatography. The data were obtained using theliquid chromatographic column with a flow rate of 0.5 mL per min and with the followingparameters: Pore size 100 A Particle Size 3.5 Internal diameter 4.6 mm Length 150 mm The food additives found in the sample resulted in the following data: ( see image for table) a.Calculate the number of plates from each peak. b.Calculate the plate height for the column. c.Retention factor for each peak.
You perform a concentration of a single component using displacement chromatography, and the following data applies: Concentration of binding sites: 4*10^(-4) M Kd for displacer: 2.0*10^(-5) M Kd for component A: 4*10^(-5) M Kd for component B: 1.5*10^(-4) M The concentration of the injected displacer is 2.0*10^(-4) M Determine and justify whether the components are part of a displacement system, and if so, provide their free and bound concentrations. Assume that the number of binding sites is the same for all components.
The figure below shows a typical separation of several fat-soluble vitamins by HPLC. The x-axis is retention time in minutes. From the graph try to estimate retention times for peaks 2 and 3. Retention time peak 2 = Retention time peak 3 = Assume that w1/2 is 0.15 minutes for peak 2 and 0.16 minutes for peak 3. Assume that tm is 2.00 minutes. How much time does gamma-tocopherol spend in the stationary phase? From tr and w1/2 find N for the peak that elutes first. From tr and w1/2 find the resolution of peaks 2 and 3 Calculate α for these peaks. If the flow rate for the column is 1.2 mL/minute what is the retention volume for peak 4?
A 200 mL water sample was treated with SPE column for pe- concentration of a certain pesticide. The final solution (24 mL) was analysed by HPLC and the .pesticide content was 1.2 ppm :The pre-concentration factor is 12.5 O 6.7 O 11.4 O 8.3
QUESTION 5 In a chromatographic separation using a polar stationary phase and a mobile phase composed of 60% hexane and 40% ethyl acetate, two compounds, benzene (peak A) and toluene (peak B), are eluted. The width of peak A at its base is 1.2 min, the width of peak B at its base is 1.3 min, and the retention time of peak A is 5.2 min and peak B is 3.5 min. What is the resolution (Rs) between these two peaks and is it acceptable? R = 1.36. It is not an acceptable resolution. R = 0.74. It is an acceptable resolution. R = 1.36. It is an acceptable resolution. R = 0.74. It is not an acceptable resolution.
The data in the following table were extracted from a gas chromatographic analysis of a two- component mixture. Air nonane decane Retention time (s) 5 26 32 Peak width, baseline (s) 5 4 (i) Calculate the capacity factor, k, for the two compounds. (ii) Calculate the selectivity factor, a, for the two compounds.
A mixture of three components is separated using paper chromatography, and each component is analyzed using absorption. With standards containing 100 ppm of each component run simultaneously, the following data were obtained: cmpd A, 0.72 absorbance; B, 0.55; C, 0.80. For the unknown mixture, the data obtined when run under identical conditions were as follows: cmpd A, .0.15 absorbance, B, 0.84; C, 0.75. a) Calculate the relative percentage composition of the unknown mixture. b) The distances traveled by the three components were 8.5, 6.3, and 5.5 cm, respectively. Sketch the appearance of the paper chromatogram. Label each spot. c) Calculate the Rf values if the solvent front is 18 cm.