The figure shows a two-dimensional gel of a mixture of proteins A, B, and C. The vertical axis represents SDS-PAGE separation, run from top to bottom. The horizontal axis represents isoelectric focusing with a pH gradient that runs from left(high pH) to right (low pH). В A Proteins A, B, and C have isoelectric points of 8.2, 6.7, and 5.2, respectively. С Place proteins A, B, and C in order of increasing elution volume on a size exclusion column. Assume they are high pH low isoelectric focusing monomers under the conditions of separation. pH SDS-PAGE Place proteins A, B, and C in order of probable increasing elution volume in cation exchange chromatography during an increasing salt gradient run at pH 5.
The figure shows a two-dimensional gel of a mixture of proteins A, B, and C. The vertical axis represents SDS-PAGE separation, run from top to bottom. The horizontal axis represents isoelectric focusing with a pH gradient that runs from left(high pH) to right (low pH). В A Proteins A, B, and C have isoelectric points of 8.2, 6.7, and 5.2, respectively. С Place proteins A, B, and C in order of increasing elution volume on a size exclusion column. Assume they are high pH low isoelectric focusing monomers under the conditions of separation. pH SDS-PAGE Place proteins A, B, and C in order of probable increasing elution volume in cation exchange chromatography during an increasing salt gradient run at pH 5.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Proteins A, B, and C have isoelctric points of 8.2, 6.7, and 5.2 respectively.
Place proteins A, B, and C in order of increasing volume on a size exclusion column.
Then place the proteins in order of probable increasing elution volume in cation exchange chromatography during an increasing salt graditend run at pH 5.
Transcribed Image Text:The figure shows a two-dimensional gel of a mixture of
proteins A, B, and C.
The vertical axis represents SDS-PAGE separation, run from
top to bottom. The horizontal axis represents isoelectric
focusing with a pH gradient that runs from left(high pH) to
right (low pH).
В
A
Proteins A, B, and C have isoelectric points of 8.2, 6.7, and
5.2, respectively.
С
Place proteins A, B, and C in order of increasing elution
volume on a size exclusion column. Assume they are
high
pH
low
isoelectric focusing
monomers under the conditions of separation.
pH
SDS-PAGE
Transcribed Image Text:Place proteins A, B, and C in order of probable increasing
elution volume in cation exchange chromatography during
an increasing salt gradient run at pH 5.
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