Tutor, please help me with clear explanation. asap The estrogen receptor is an important protein to mediate the actions of estrogens in targetcells. It is hypothesized that estrogen receptor will form a dimer in the presence of estrogens.You are provided with the following materials:(i) The cDNA clone of the estrogen receptor;(ii) Yeast expression vector (selection marker: HIS3) with a partial gene encoding the DNAbinding domain of the GAL4 transcriptional factor;(iii) Yeast expression vector (selection marker : TRP1) with a partial gene encoding theactivation domain of the GAL4 transcriptional factor;(iv) Reporter yeast strain (defective in his3, trp1, ura3) with GAL1 promoter-URA3 and GAL1promoter-lacZ (GAL4 can activate GAL1 promoter);(v) Estrogens(vi) All chemicals for yeast cultivation and transformation;(vii) Chemicals for mutagenesis; and(viii) All other common laboratory chemicals and facilities.(a) Using yeast two-hybrid technology, design an experiment to test this hypothesis.(b) Suppose the hypothesis is correct, using a similar system, identify the amino acidresidue(s) on the estrogen receptor that is/are essential for the binding of estrogens.

Name: Tutor, please help me with clear explanation. asap The estrogen recept
Uploaded: 2024-03-20T06:42:41.000Z
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Description: Tutor, please help me with clear explanation. asap The estrogen receptor is an important protein to mediate the actions of estrogens in targetcells. It is hypothesized that estrogen receptor will form a dimer in the presence of estrogens.You are prov