The diagram below represents the tryptophan operon with the trp leader MRNA transcript enlarged to represent the AUG transiation start codon, two consecutive tryptophan amino acid codons (UGGUGG), and 4 regions (1, 2, 3, and 4) that base pair to form different hairpin-loop structures in the MRNA leader region. What would happen in this mRNA leader region when cells encounter very low levels of tryptophan in its environment? Leader region trpE trpD trpC trpB trpA DNA 5' Transcription trp leader sequence mRNA UGGUGG (tryptophan codons) AUG UUUUUU O The translating ribosome would stall at the two tryptophan codons, causing the formation of a hairpin-loop botween regions 3 and 4 to promote transcription of the trp operon OThe translating ribosome would stall at the two tryptophan codons causing formation of hairpin-loop between regions 2 and 3, which functions as an ant-tarminator of transcription. O The translating ribosome would stall at the two tryptophan codons causing formation of hairpin-locp between regions 1 and 2, which functions as a torminator of transcriotion O The translating ribosome would stall at the UUUUUU sequence to prevent the formation of a transcription termination loop The translating ribosomo would stall at the two tryptophan codons causing formation of hairpin-loop between rogions 2 and 3, which functions as an anti-terminator of transcnption
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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