The decolorizer used in the Gram Stain, and the decolorizer used in the Acid Fast Stain, are the same

Q: Explain the reasons you would use a negative stain?

A: In a negative stain the slide is actually stained. This is because the negatively charged stain…

Q: Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram…

A: Gram staining is the common procedure followed to distinguish the bacteria based on their cell wall.…

Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: Explain the significance of crystal violet, iodine, Grams decolorizer, and safrin make in Gram…

A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…

Q: hich Is The More Important Step In Gram Stain?

A: Hans Christian Gram invented the Gram stain in 1884. (Gram,1884).

Q: Why is Iodine used in the Gram Staining procedure?

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: Which of the following are true about a differential stain. There may be more than one answer!…

A: A microscopic organism made of one cell is referred to as a prokaryote. Prokaryotic organisms do…

Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…

A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…

Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: Choose the 4 most correct steps from the list below for a simple positive stain and organize them…

A: The above question is related to the laboratory technique of bacterial staining. Staining is a…

Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…

A: Option b

Q: Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining…

A: The correct option is . A Gram's Staining procedure

Q: Select all that apply to a direct simple stain:    1. the dye is acidic or negatively charged and…

A: Gram staining is used to examine the shape of the bacterial cells; if they are contaminated.…

Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…

A: To answer this question first, we need to understand what is gram staining and what are the steps of…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…

A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…

Q: The bacterial cells in the image below were stained with a high concentration of carbolfuschin for…

A: Carbol fuchsin, carbolfuchsin, or carbol fuchsin is basically a mixture of "phenol" and basic…

Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…

A: The acid-fast staining technique is a differential staining technique. The bacteria which get…

Q: Explain the importance of rinsing with water every after each step in gram staining.

A: Introduction : Bacteria can be categorised in a number of ways depending on their morphology,…

Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…

A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…

Q: You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the…

A: Gram negative bacteria: these bacteria are enclosed in hard capsule and don't retain the crystal…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique used for staining the microbial samples. It was performed by the…

Q: The acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______.…

A: The staining procedures used to differentiate the types of bacteria are known as a differential…

Q: Additional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain,…

A: Staining is performed to enhance the sample present on a slide against its backdrop in such a way…

Q: What's the difference between the gram stain of Bacillus megaterium and Bacillus Subtilis?

A: Bacillus megaterium and Bacillus subtilis are both gram-positive, rod-shaped bacteria. However,…

Q: Why is it essential that the primary stain and the counterstain be contrasting colors in…

A: The gram staining is used to distinguish between gram negative and gram positive bacteria. It was…

Q: Which step of the gram stain procedure is most critical?why?

A: Gram staining is one of the most common laboratories test to identify the different bacterial…

Q: What is the purpose of iodine in the Gram stain?   To act as a counterstain   None of the other…

A: There are various staining techniques that are used to stain different cellular structures. The Gram…

Q: You mistakenly confused the primary stain and counter stain with crystal violet, how does you…

A: Gram staining is a widely used laboratory technique in microbiology and bacteriology to…

Q: Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and…

A: Bacteria are small, microscopic and unicellular organisms that can live in variety of environment.…

Q: This part of the staining process helps the primary stain to remain in the cell during…

A: Staining of biological samples is a very useful process for detailed observation and measurement of…

Q: List the primary stain, secondary stain, mordant, and decolorizer for the gram stair and write them…

A: The strains in the plate is give on two strains the primary strain and secondary strain. The primary…

Q: Is the gram stain significant to microorganisms other than bacteria? explain

A: The Gram stain is primarily used to differentiate bacteria into two broad categories: Gram-positive…

Q: Answer the following questions  What is the purpose of the 95% ethanol step in the Gram staining…

A: The gram staining technique is used to differentiate between the gram positive and gram negative…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…

Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: hat stain technique must this be? What is used to stain the background? What is used to stain the…

A: Capsules have a low staining ability with conventional dyes. Typically, capsule staining procedures…

Q: It is expected that amoxicillin would be more effective against Gram-positive bacteria.  Explain…

A: Amoxicillin is a beta-lactam antibiotic that functions by attaching itself to the membrane proteins…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.