Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation (Leifson staining technique)
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Specific Dyes used in flagella stain:
- A wet-mount procedure (Ryu method)
- Dried-smear preparation (Leifson staining technique)
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- Principle/ technique used in Flagella Stain Method/ Preparation/ Procedure of Flagella StainDefine the term gram staining.What stain technique must this be? What is used to stain the background? What is used to stain the bacterial cell (in our method)? What structure is the clear (white) layer? Do we heat fix this? Capsule stain, No heat fixation, crystal violet, copper sulfate,
- This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantWhy is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Describe the bacteria observed in different staining procedures. Please refer to the photo - Gram Stain - Acid fast stain
- What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple stain, and differential stain. please provide minimum of 400 wordsIs it possible to perform double or triple staining using fluorescent dyes? Explain.