**Question: The catalytic mechanism of bovine pancreatic RNase A relies upon acid-base catalysis involving the amino acid ________.**- ○ imidazole - ○ lysine- ○ histidine- ○ aspartic acid- ○ glutamic acid ### Enzyme Kinetic Constants and Drug EffectsYou are studying the effects of a new drug on the activity of the enzyme elevated in response to a disease. The results of your studies show the drug is acting as a pure noncompetitive inhibitor. Which of the following changes to the enzyme kinetic constants will occur from this drug's actions? (Choose all correct answers)- [ ] Km will increase- [ ] Km will remain unchanged- [ ] Vmax will decrease- [ ] Vmax will remain unchanged- [ ] k_cat (turnover number) will decrease

Bovine pancreatic RNase is a nuclease Enzyme that catalyses the cleavage of RNA. The protein…

Answered: The catalytic mechanism of bovine…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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TLCK and TPCK both use Histadine residues in the active site as inhibitors of serine proteases. Why is TLCK specific for trypsin and TPCK is specific for chymotrypsin?
The enzyme cytidine deaminase catalyzes the conversion of cytidine to uridine. Cytidine deaminase catalyzes the reaction through an addition of water across the cytidine 3,4-bond, forming a tetrahedral intermediate followed by the elimination of NH3 to form the product uridine. This is like the addition-elimination mechanism that we studied for adenosine deaminase. Cytidine deaminase НО. NH₂ N пOH OH cytosine R + H₂O cytidine The Km value for the substrate cytidine is 2.5 × 10-4 M, and the K; for competitive inhibition by the product uridine is 2.5 × 10-³ M. N R A reduced derivative of the product, 3,4,5,6-tetrahydrouridine was shown to be a fully reversible competitive inhibitor with a Ki of 2.4 x 10-7 M, a value approximately 10,000 times lower than that of the product uridine. NH₂ NH uridine HO. N R R = D-ribose HOH OH uridine 3NH uracil ring numbering H NH H H + NH3 H OH H 3,4,5,6-tetra- hydrouridine a.) Draw a structure of the intermediate that we predict to form during the…
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The catalytic mechanism of bovine pancreatic RNase A relies upon acid-base catalysis involving the amino acid imidazole lysine histidine aspartic acid Oglutamic acid