The amino acid sequence of a biologically active dodecapeptide was determined by treating the dodecapeptide with chemical and enzymatic reagents. The results obtained are given below:Amino acid composition: F. G(2), I, K, L(2), Q, R, T, V, WHydrazine Treatment of the dodecaptide gave underivatized GTrypsin digestion yielded 3 fragments:Fragment A: F, K, L, T Fragment B: G, VFragment C: G,I,L,Q,R,W (Edman reaction of this fragment gave Pth-IChymotrypsin digestion yielded 3 fragments:Fragment D: G(2), L,R,V (Edman reaction of this fragment gavePth-L)Fragment E: F,TFragment F: I,K,L,Q,W (Edman reaction of this reaction gave Pth-L.What is amino acid sequence of the dodecapeptide?

Dodecapeptide is a peptide which is composed of twelve amino acid residues. Edman degradation method…

Answered: The amino acid sequence of a…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

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Chapter1: The Human Body: An Orientation

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The amino acid sequence of a biologically active dodecapeptide was determined by treating the dodecapeptide with chemical and enzymatic reagents. The results obtained are given below:

Amino acid composition: F. G(2), I, K, L(2), Q, R, T, V, W
Hydrazine Treatment of the dodecaptide gave underivatized G
Trypsin digestion yielded 3 fragments:

Fragment A: F, K, L, T Fragment B: G, V

Fragment C: G,I,L,Q,R,W (Edman reaction of this fragment gave Pth-I
Chymotrypsin digestion yielded 3 fragments:
Fragment D: G(2), L,R,V (Edman reaction of this fragment gave

Pth-L)

Fragment E: F,T

Fragment F: I,K,L,Q,W (Edman reaction of this reaction gave Pth-L.

What is amino acid sequence of the dodecapeptide?

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